Gene Rv0939
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism, possibly in a degradation pathway. |
| Product | Possible bifunctional enzyme: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HHDD isomerase) + cyclase/dehydrase |
| Comments | Rv0939, (MTCY10D7.35c), len: 644 aa. Possible bifunctional enzyme, including 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase activity, and cyclase/dehydrase activity. N-terminal part similar to many isomerases e.g. NP_343861.1|NC_002754 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Sulfolobus solfataricus (318 aa); NP_068932.1|NC_000917 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (hpcE-1) from Archaeoglobus fulgidus (324 aa), FASTA scores: opt: 400, E(): 5.8e-15, (33.9% identity in 289 aa overlap); etc. And C-terminal part highly similar to many cyclases/dehydrases e.g. AAK61721.1|AY033994 cyclase-like protein from Streptomyces aureofaciens (305 aa); CAC44204.1|AL593842 cyclase from Streptomyces coelicolor (297 aa), FASTA scores: opt: 375, E(): 2.7e-26, (35.6% identity in 284 aa overlap); NP_343860.1|NC_002754 putative Cyclase/dehydrase from Sulfolobus solfataricus (308 aa); etc. Also similar to Rv2993c hypothetical protein from Mycobacterium tuberculosis. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1048412 | 1050346 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0939|Rv0939
MKWVTYRSDHGERTGVLSGDAIYAMPPDVSLLDLVGRGADGLRTAGERAVRSPAAVVALDEVTLAAPIPRPPSIRDSLCFLDHMRNCQEAMGGGRVLMDTWYRIPAFYFACPSTVLGPYDDAPTAPGSAWQDFELEIAAVIGTSGKDLTVEQAERSIIGYTIFNDWSARDLQMLEGQLRIGQAKGKDSGITLGPYLVTPDELEPYCRGGKLSLRVIALVNGTVIGSGSTAQMDWSFGEVIAYASRGVTLTPGDVFGSGTVPTCTLVEHLRPPESFPGWLHDGDVVTLQVEGLGETRQTVRTSGTPFPLALRPNPDAEPDRRGVNPAPTRVPFTRGLHEVADRVWAWTLPDGGYGFSNAGLVAGDGASLLVDTLFDLALTREMLAAMKPVTERAPITDALITHSNGDHTHGTQLLDRSVRIIAAKGTSEEIEHGPAPEMLARIQTADLGPVATRYLRDRFGHFDFSGIKLRNADLTFDRDLAIELGGRRVDLLNLGPAHTTADSVVHVADAGVLFAGDLLFIGCTPIVWAGPIANWVAACDAMIALDAPTVVPGHGPVTGPDGIRAVRGYLAHIAEQAEAAYRKGLSLPEAVETIDLGEYASWLDSERVVVNVYQRYRELDPDTPRQDLLALLVMQAEWAARHCT
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
