Gene Rv0982
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Sensor part of a two component regulatory system (MPRAB system) |
| Product | Two component sensor kinase MprB |
| Comments | Rv0982, (MTV044.10), len: 504 aa. MprB, two component sensor kinase, probable transmembrane protein (see citation below), equivalent to AL035500|MLCL373_16|NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa), FASTA score: (81.0% identity in 521 aa overlap). Also highly similar to others (especially in C-terminal part) e.g. AAG36760.1|AF119221_2|AF119221 sensor kinase from Corynebacterium glutamicum (455 aa); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (481 aa); X58793|SLCUTRS_2 sensor kinase from S.lividans (414 aa), FASTA scores: opt: 451, E(): 4.2e-21, (36.0% identity in 303 aa overlap); P30847|BAES_ECOLI sensor protein from Escherichia coli (467 aa), FASTA scores: opt: 412, E(): 1.3e-18, (30.4% identity in 336 aa overlap); etc. Also similar in C-terminal region to C-terminus of Rv0902c|Z73101|MTCY31_33 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 423, E(): 2.6e-19, (28.4 identity in 462 aa overlap). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006). |
| Functional category | Regulatory proteins |
| Proteomics | Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | DNA microarrays detect expression in M. tuberculosis H37Rv in vivo (in BALB/c and SCID mice) but not in vitro (in 7H9 medium) (See Talaat et al., 2004). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Growth and cytotoxicity of M. tuberculosis H37Rv Rv0982 mutant in THP-1 cells is comparable to wild-type (See Guinn et al., 2003). M. tuberculosis H37Rv mprA|Rv0981-mprB|Rv0982 mutant has increased survival in vitro in the presence of 0.05% SDS; growth rate in human monocytes is higher than wild-type (See Pang et al., 2007). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1097508 | 1099022 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0982|mprB
MWWFRRRDRAPLRATSSLSLRWRVMLLAMSMVAMVVVLMSFAVYAVISAALYSDIDNQLQSRAQLLIASGSLAADPGKAIEGTAYSDVNAMLVNPGQSIYTAQQPGQTLPVGAAEKAVIRGELFMSRRTTADQRVLAIRLTNGSSLLISKSLKPTEAVMNKLRWVLLIVGGIGVAVAAVAGGMVTRAGLRPVGRLTEAAERVARTDDLRPIPVFGSDELARLTEAFNLMLRALAESRERQARLVTDAGHELRTPLTSLRTNVELLMASMAPGAPRLPKQEMVDLRADVLAQIEELSTLVGDLVDLSRGDAGEVVHEPVDMADVVDRSLERVRRRRNDILFDVEVIGWQVYGDTAGLSRMALNLMDNAAKWSPPGGHVGVRLSQLDASHAELVVSDRGPGIPVQERRLVFERFYRSASARALPGSGLGLAIVKQVVLNHGGLLRIEDTDPGGQPPGTSIYVLLPGRRMPIPQLPGATAGARSTDIENSRGSANVISVESQSTRAT
Bibliography
- Zahrt TC et al. [2001]. Mycobacterium tuberculosis signal transduction system required for persistent infections. Mutant Function Regulation
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Zahrt TC et al. [2003]. Functional analysis of the Mycobacterium tuberculosis MprAB two-component signal transduction system. Function
- Talaat AM et al. [2004]. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Transcriptome
- Guinn KM et al. [2004]. Individual RD1-region genes are required for export of ESAT-6/CFP-10 and for virulence of Mycobacterium tuberculosis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- He H et al. [2006]. MprAB is a stress-responsive two-component system that directly regulates expression of sigma factors SigB and SigE in Mycobacterium tuberculosis. Function Regulation
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Pang X et al. [2007]. Evidence for complex interactions of stress-associated regulons in an mprAB deletion mutant of Mycobacterium tuberculosis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
