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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionSensor part of a two component regulatory system (MPRAB system)
ProductTwo component sensor kinase MprB
CommentsRv0982, (MTV044.10), len: 504 aa. MprB, two component sensor kinase, probable transmembrane protein (see citation below), equivalent to AL035500|MLCL373_16|NP_301251.1|NC_002677 putative two-component system sensor kinase from Mycobacterium leprae (519 aa), FASTA score: (81.0% identity in 521 aa overlap). Also highly similar to others (especially in C-terminal part) e.g. AAG36760.1|AF119221_2|AF119221 sensor kinase from Corynebacterium glutamicum (455 aa); CAB89748.1|AL354616 putative two-component histidine kinase from Streptomyces coelicolor (481 aa); X58793|SLCUTRS_2 sensor kinase from S.lividans (414 aa), FASTA scores: opt: 451, E(): 4.2e-21, (36.0% identity in 303 aa overlap); P30847|BAES_ECOLI sensor protein from Escherichia coli (467 aa), FASTA scores: opt: 412, E(): 1.3e-18, (30.4% identity in 336 aa overlap); etc. Also similar in C-terminal region to C-terminus of Rv0902c|Z73101|MTCY31_33 from Mycobacterium tuberculosis (446 aa), FASTA scores: opt: 423, E(): 2.6e-19, (28.4 identity in 462 aa overlap). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006).
Functional categoryRegulatory proteins
ProteomicsPredicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
TranscriptomicsDNA microarrays detect expression in M. tuberculosis H37Rv in vivo (in BALB/c and SCID mice) but not in vitro (in 7H9 medium) (See Talaat et al., 2004).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Growth and cytotoxicity of M. tuberculosis H37Rv Rv0982 mutant in THP-1 cells is comparable to wild-type (See Guinn et al., 2003). M. tuberculosis H37Rv mprA|Rv0981-mprB|Rv0982 mutant has increased survival in vitro in the presence of 0.05% SDS; growth rate in human monocytes is higher than wild-type (See Pang et al., 2007).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS10975081099022+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0982|mprB
MWWFRRRDRAPLRATSSLSLRWRVMLLAMSMVAMVVVLMSFAVYAVISAALYSDIDNQLQSRAQLLIASGSLAADPGKAIEGTAYSDVNAMLVNPGQSIYTAQQPGQTLPVGAAEKAVIRGELFMSRRTTADQRVLAIRLTNGSSLLISKSLKPTEAVMNKLRWVLLIVGGIGVAVAAVAGGMVTRAGLRPVGRLTEAAERVARTDDLRPIPVFGSDELARLTEAFNLMLRALAESRERQARLVTDAGHELRTPLTSLRTNVELLMASMAPGAPRLPKQEMVDLRADVLAQIEELSTLVGDLVDLSRGDAGEVVHEPVDMADVVDRSLERVRRRRNDILFDVEVIGWQVYGDTAGLSRMALNLMDNAAKWSPPGGHVGVRLSQLDASHAELVVSDRGPGIPVQERRLVFERFYRSASARALPGSGLGLAIVKQVVLNHGGLLRIEDTDPGGQPPGTSIYVLLPGRRMPIPQLPGATAGARSTDIENSRGSANVISVESQSTRAT
      
Bibliography