Gene Rv1069c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved protein |
| Comments | Rv1069c, (MTV017.22c), len: 587 aa. Conserved protein, hydrophobic regions in N-terminal domain. Similar in part to O07136|B1306.04C B1306.04c protein from Mycobacterium leprae (89 aa), FASTA scores: opt: 229, E(): 1.3e-07, (54.2% identity in 72 aa overlap). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS; predicted integral membrane protein (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1192510 | 1194273 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1069c|Rv1069c
MTEPAAATTTNASDEPATGAEQAVDTAATPQTPEPQPIRSTWWIRHYTFTGTAMGLVFVWFSMTPSLLPRGPLFQGLVSGICGAFGYGLGVFAVWLVRYMRSHNSSPPPPRWAWPPLIAVGAVGMVGMAVQFHVWQDDVRDLMGVEHLRWYDYPLAAALSLVVLFTLVEIGQFIRWLFRFLVGQVDRIAPFRVSAAIVVVLLVVLTITLLNGVVLKFAMNSMNSTFAAVNNEMNPDSAPPKTPLRSGGPGSLVSWESLGHQGRIFVHSGPTIADLTAFNGTPAVEPIRTYAGLNSADGIMATAELAARELARTGGLRRAVVAVATSTGTGWINEAEASALEYMYNGDTAIVSMQYSFLPSWLSFLVDKENARHAGEALFEAVDKLIRQLPESQRPKLVVFGESLGSFGGEAPFMNLNNILARTDGALFSGPTFNNTVWNSLTANRDAGSPQWLPIYDDGRNVRFVARARDLQRPDAPWGRPRVVYLQHASDPIAWWTPRLLFREPDWLREQRGYDVLPQTRWIPVVTFVQVSADMAVATHVPDGHGHRYVATVADGWAAVLSPPGWTQQKTERLQPLLHANAKPFGS
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
