Gene Rv1128c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv1128c, (MTCY22G8.17c), len: 451 aa. Conserved hypothetical protein, in REP13E12 degenerate repeat, highly similar to several Mycobacterium tuberculosis proteins in REP13E12 repeats e.g. Rv1148c, Rv1945, Rv3467, etc. |
| Functional category | Insertion seqs and phages |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 96h of starvation (see citation below). DNA microarrays show lower level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1251617 | 1252972 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1128c|Rv1128c
MCSTREEITEAFASLATALSRVLGLTFDALTTPERLALLEHCETARRQLPSVEHTLINQIGEQSTEEELGGKLGLTLADRLRITRSEAKRRVAEAADLGQRRALTGEPLPPLLTATAKAQRHGLIGDGHVEVIRAFVHRLPSWVDLKTLEKAERDLAKQATQYRPDQLAKLAARIMDCLNPDGDYTDEDRARRRGLTLGKQDVDGMSRLSGYVTPELRATIEAVWAKLAAPGMCNPEQKAPCVNGAPSKEQARRDTRSCPQRNHDALNAELRSLLTSGNLGQHNGLPASIIVTTTLKDLEAAAGAGLTGGGTILPISDVIRLARHANHYLAIFDRGKALALYHTKRLASPAQRIMLYAKDSGCSAPGCDVPGYYCEVHHVTPYAQCRNTDVNDLTLGCGGHHPLAERGWTTRKNAHGDTEWLPPPHLDHGQPRVNTFHHPEKLLADDEGDP
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
