Gene Rv1146
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown. Thought to be involved in fatty acid transport. |
| Product | Probable conserved transmembrane transport protein MmpL13b |
| Comments | Rv1146, (MTCI65.13), len: 470 aa. Probable mmpL13b, conserved transmembrane transport protein (see citation below), member of RND superfamily, showing some similarity to putative Mycobacterial and Streptomyces membrane proteins e.g. Q53902|C40046 antibiotic transport-associated protein from Streptomyces coelicolor (711 aa), FASTA scores: opt: 193, E(): 2.1e-05, (28.9% identity in 394 aa overlap); etc. Could be in frame with previous ORF mmpL13A|Rv1145, but no sequence error apparent to account for this; sequence is identical in M. tuberculosis strain CDC1551, and Mycobacterium bovis strain AF2122/97. Belongs to the MmpL family. |
| Functional category | Cell wall and cell processes |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1273355 | 1274767 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1146|mmpL13b
VATVAFVATASIVITPAAIVLLGPRLDALDVRRLVRRLLGRPDPVHKPVKQLFWYRSSKFVMRRWLPVGTAVVALLVLLGLPFLSVKWGFPDDRVLPRSASARQVGDILRDDFGHDPATQIPIVVPDARGLGPVELDSYAAELSRVPDVSAVAAPTGTFVDGSWVGTPRGATGLAEGSAFLTVSSTAPLFSRASDIQLKRLHQVAGPAGRSVVMAGVAQVNRDSVDAVTDRLPMVLGLIAAITYVLLFLLTGSVVLPAKALVCNVLSLTAAFGALVWIFQEGHFGALGTTPSGTLVANMPVLLFCIAFGLSMDYEVFLVSRIREYWLESGAARPARRSVAEVHAANDESVALGVARTGRVITAAALVMSMSFAALIAAHVSFMRMFGLGLTLAVAADATLVRMVVVPAFMHVTGRWNWWAPRPLAWLHERFGVSEAAEPVSRRRSHAGGLGKIAGRSDGQTIPASLTRNG
Bibliography
- Tekaia F et al. [1999]. Analysis of the proteome of Mycobacterium tuberculosis in silico. Secondary Function
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
