Gene Rv1153c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but supposedly involved in lipid metabolism |
| Product | Probable O-methyltransferase Omt |
| Comments | Rv1153c, (MTCI65.20c), len: 282 aa. Probable omt, O-methyltransferase, similar to TCMP_STRGA|P39887 Tetracenomycin polyketide synthesis O-methyltransferase tcmP from Streptomyces glaucescens (270 aa), FASTA scores: opt: 368, E(): 1.7e-17, (31.3% identity in 233 aa overlap). |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1279998 | 1280846 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1153c|omt
VSAHKPAKQRVALTGVSETALLTLNARAAEARRRDAIIDDPMAVALVESIDFDFAKFGPTGQGFALRARAFDMAAQHYLDQHPAATVVALAEGLQTSFWRLDVAIPGGQFRWLTVDLPPIVDLRTRLLPSSPRVSVCAQSALDYSWMDSVDPAGGVFITAEGLLMYLQPEQALGLIAQCAQTFPGGQMLFDLPPRWFAGWSRLGLRTSLRYKVPRMPFSMSVAQAADLVNKVPGVVAVRDLRVPPGRGLWVNMALSTVYRLPVFDPLRPCLTLLEFSRPARG
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
