Gene Rv1318c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to play an essential role in regulation of cellular metabolism by catalysing the synthesis of a second messenger, camp [catalytic activity: ATP = 3',5'-cyclic AMP + pyrophosphate]. |
| Product | Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase) |
| Comments | Rv1318c, (MTCY130.03c), len: 541 aa. Possible adenylate cyclase. Some similarity at the c-terminus to CYAA_RHIME|P19485 adenylate cyclase from Rhizobium meliloti (193 aa), FASTA scores, opt: 270, E(): 2.5e-11, (28.8% identity in 184 aa overlap); similar to other mycbacterium tuberculosis putative adenylate cyclases e.g. Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 2505, E(): 0, (71.0% identity in 534 aa overlap), also similar to Rv1320c|MTCY130.05c (567 aa), FASTA scores, opt: 2423, E(): 0, (68.7% identity in 534 aa overlap). N-terminus is hydrophobic. Belongs to adenylyl cyclase class-3 family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1479199 | 1480824 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1318c|Rv1318c
MSAKKSTAQRLGRVLETVTRQSGRLPETPAYGSWLLGRVSESQRRRRVRIQVMLTALVVTANLLGIGVALLLVTIAIPEPSIVRDTPRWLTFGVVPGYVLLALALGSYALTRQTVQALRWAIEGRKPTREEERRTFLAPWRVAVGHLMFWGVGTALLTTLYGLINNAFIPRFLFAVSFCGVLVATATYLHTEFALRPFAAQALEAGPPPRRLAPGILGRTMVVWLLGSGVPVVGIALMAMFEMVLLNLTRMQFATGVLIISMVTLVFGFILMWILAWLTATPVRVVRAALRRVERGELRTNLVVFDGTELGELQRGFNAMVAGLRERERVRDLFGRHVGREVAAAAERERSKLGGEERHVAVVFIDIVGSTQLVTSRPPADVVKLLNKFFAIVVDEVDRHHGLVNKFEGDASLTIFGAPNRLPCPEDKALAAARAIADRLVNEMPECQAGIGVAAGQVIAGNVGARERFEYTVIGEPVNEAARLCELAKSRPGKLLASAQAVDAASEEERARWSLGRHVKLRGHDQPVRLAKPVGLTKPRR
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
