Gene Rv1358
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism |
| Product | Probable transcriptional regulatory protein |
| Comments | Rv1358, (MTCY02B10.22), len: 1159 aa. Probable transcriptional regulatory protein, some similarity to AFSR_STRCO|P25941 regulatory protein afsr from Streptomyces coelicolor (993 aa), FASTA scores: opt: 210, E(): 5.5e-06, (27.5% identity in 739 aa overlap). Similar also to Rv0890C|MTCY31.18c (65.5% identity in 884 aa overlap) and to Rv1359|MTCY02B10.23 (43.7% identity in 197 aa overlap). Contains PS00017 ATP/GTP-binding site motif A, PS00622 Bacterial regulatory proteins, luxR family signature. Helix turn helix motif present at aa 1116-1137, (Score 1291, +3.59 SD). |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1526612 | 1530091 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1358|Rv1358
MFLSAPAFRVEPTRSRHSALRWARHRRFADGPRWQMLRSLQIADQIARTGHMPVRRLDLIWISARNAARRELDLGVAALVEAVTLLTADVEGSTRLSQTRLNELAADYPTLDQNISEAVAAHGGVTRPVDQEVGSGLVVAFLRAGDAIACALELQLSTLAPMRPRVGVHTGDVRLRGDGTITGSAINESACLRDLAHEGQTLLSAATGDLVIDQLPANTWLTDVGKYPLRGLHRQERVIQLCHRDLRNEFPPLRMSVGNRSSLPAQFTTFVGRDAQINEVQEVLTNYRLVTLRGEGGVGKTRLAIQIAAASEFRDGLCFVDLAPIADPGMVSTTAAHALGLIDRPGSSTFDTLSHAIGNCHMLMVLDNCEHVLDACAELVVELLGACPELSILATSRESIGVTGEVTWVVPSLSPANEAIQLFTERARLVQPNFEIVADNFDAVSEICRRLDGMPLAIELAAARLRSLSPNEIANSLDDRFRLLTGGARSTVQRQQTLRASMDWSYALLTDTERILFRRLAVFVGGFDLTAASEVAAAGGDDFVERYSVLDQLTLLVDKSLVVAEESRGSTRYRLLETVRQYALEKLNESEEIDGVRARHRTHYATMAAGLNVPASTDYEQRLLQAEAEIDNLRAAFTWSRGNGDIAAALQLASALQPLWSQGRMREGLAWLESILEREGDNHLVPAGVWARALAEKVILKAWPATSPMGAPDIVAQAHHALALARDAGDCAVLARALVACGCGSGCDTEAAQPYFAEAIELARAINDEWTLSQIDYWQVVGIFISGQPIPLRAAAEQARELADSIGNRFVSRQCRLFACLAQIWEGDANGALALSRDVTAEAEVANDVVTKVLGLYVEAMALSYIGDSAARTIAGAALEAATELGGIYQDLGYGAITRAALAAGDVAAIEASEASWDLRNQHNVVTAHHELMAQAALVRGDVTTARRFADEAVLASTGWHLMMALIARARVAIAQDELGKARDDAHAAVACGVGVQTYLAMPDALELLAGLAGEAGNHGQAVRLFGAAAAQRQRTGEVRHKIWDAGYEAATAALRDAMGDEDFTAAWAEGAAAPLDEAIAYAQRGRGERKRPSNGWDALTPAEHKIVKLVTEGLVTKDIAARLFVSPRTVQTHLTHIYTKLDVTSRVQLVQEAAQHST
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
