Gene Rv1395
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism |
| Product | Transcriptional regulatory protein |
| Comments | Rv1395, (MTCY21B4.12), len: 344 aa. Transcriptional regulatory protein (see citation below), similar to many e.g. URER_PROMI|Q02458 urease operon transcriptional activator from Proteus mirabilis (293 aa), FASTA scores: E():1.5e-08, (41.7% identity in 84 aa overlap); YHIX_ECOLI|P37639 hypothetical transcriptional regulatory protein from Escherichia coli (274 aa), FASTA scores: opt: 238, E(): 3.5e-09, (27.3% identity in 249 aa overlap); and G296916|X68281 possible virulence-regulating protein from Mycobacterium tuberculosis (339 aa), FASTA scores: opt: 228, E(): 1.9e-08, (27.0% identity in 278 aa overlap). Helix turn helix motif present, aa 261-282 (+4.68 SD). Belongs to the AraC/XylS family of transcriptional regulators. 3' part corrected since first submission (-14 aa). Experimentally shown to induce the expression of the cytochrome P450 gene (Rv1394c) and represses its own transcription. |
| Functional category | Regulatory proteins |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1571047 | 1572081 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1395|Rv1395
VGHLPPPAEVRHPVYATRVLCEVANERGVPTADVLAGTAIEPADLDDPDAVVGALDEITAVRRLLARLPDDAGIGIDVGSRFALTHFGLFGFAVMSCGTLRELLTIAMRYFALTTMHVDITLFETADDCLVELDASHLPADVRGFFIERDIAGIIATTTSFALPLAAKYADQVSAELAVDAELLRPLLELVPVHDVAFGRAHNRVHFPRAMFDEPLPQADRHTLEMCIAQCDVLMQRNERRRGITALVRSKLFRDSGLFPTFTDVAGELDMHPRTLRRRLAEEGTSFRALLGEARSTVAVDLLRNVGLTVQQVSTRLGYTEVSTFSHAFKRWYGVAPSEYSRRG
Bibliography
- Camacho LR, Ensergueix D, Perez E, Gicquel B and Guilhot C [1999]. Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature-tagged transposon mutagenesis. Mutant
- Recchi C et al. [2003]. Mycobacterium tuberculosis Rv1395 is a class III transcriptional regulator of the AraC family involved in cytochrome P450 regulation. Mutant Product Biochemistry Function
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
