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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionFunction unknown, but supposed involvement in lipid degradation.
ProductPossible long-chain-fatty-acid--CoA ligase FadD12 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase)
CommentsRv1427c, (MTCY493.27), len: 535 aa. Possible fadD12, long-chain-fatty-acid-CoA synthetase, similar to many e.g. NP_302632.1|NC_002677 acyl-CoA synthase from Mycobacterium leprae (548 aa); AAD01929.2|AF031419 putative long-chain-fatty-acid--CoA ligase from Pseudomonas putida (565 aa); NP_419782.1|NC_002696 putative long-chain-fatty-acid--CoA ligase from Caulobacter crescentus (530 aa); PC60_YEAST|P38137 yeast peroxisomal-coenzyme A synthetase (543 aa), FASTA scores: opt: 507, E(): 2.9e-25, (30.4% identity in 365 aa overlap). Also similar to many M. tuberculosis proteins e.g. MTCY06A4.14 (44.8% identity in 525 aa overlap). Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family.
Functional categoryLipid metabolism
ProteomicsIdentified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS16023211603928-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1427c|fadD12
MRIRQAFGLIATMRRAGLIAPLRPDRYLRIVAAMRREGMGFTAGFAGAARRCPDRPGLIDELGTLTWRQLDERGNALAAALQALPAGPPRVVGIMCRNHRGFVDALLAVNRIGAHILLLNTSFAGPALAEVVTREGVDTVVYDEEFSATVDRALAEKPQATRIVAWTDEDHDLTVEKLVAAHAGRRPEHTGSHGKVILLTSGTTGTPKGARHSGGGIGTLKAILDRTPWRAEEVTVIVAPMFHAWGFSQLVLASSLACTIVTRRRFDPEATLDLIDRHHATGLVVVPVMFDRIMDLPAEIRNRYDGRSLRFAAASGSRMRPDVVIAFMDQFGDVIYNNYNATEAGMIATATPADLRTAPDTAGRPAEGTEIRILDQQFTEVPTGEVGTIYVRNDSQFDGYTSGAAKDFHAGFMSSGDVGYLDENGRLFVVGRDDEMIVSGGENIYPIEVEKTLATHPDVAEAAVIGVDDQQYGQRLAAFVVLKPGVSATPETLKQHVRDNLANYKVPRDIAVLDELPRGITGKILRTELQSRVGS