Gene Rv1474c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism |
| Product | Probable transcriptional regulatory protein |
| Comments | Rv1474c, (MTV007.21c), len: 187 aa. Probable transcription regulator, equivalent to AF0021|AF002133_1 transcriptional regulator from Mycobacterium avium strain GIR10 (82 aa), FASTA scores: opt: 490, E(): 6.7e-26, (92.5% identity in 80 aa overlap). Also similar to Q59431|UIDR_ECOLI UID operon repressor (GUS operon) from Escherichia coli (196 aa), FASTA scores: opt: 192, E(): 5.8e-06, (28.5% identity in 172 aa overlap). Belongs to the TetR/AcrR family of transcriptional regulators. Helix turn helix motif predicted at aa 33-54 (+3.40 SD). |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1662641 | 1663204 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1474c|Rv1474c
VPKVSEDHLAARRRQILDGARRCFAEYGYDKATVRRLEQAIGMSRGAIFHHFRDKDALFFALAREDTERMAAVASREGLIGVMRDMLAAPDQFDWLATRLEIARKLRNDPDFSRGWAERSAELAAATTDRLRRQKQANRVRDDVPSDVLRCYLDLVLDGLLARLASGEDPQRLAAVLDLVENSVRRS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
