Gene Rv1521
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involvement in lipid degradation. |
| Product | Probable fatty-acid-AMP ligase FadD25 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) |
| Comments | Rv1521, (MTCY19G5.07), len: 583 aa. Probable fadD25, fatty-acid-AMP synthetase, highly similar to many e.g. P71495|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2486, E(): 0, (63.4% identity in 584 aa overlap); NP_301232.1|NC_002677 acyl-CoA synthetase from Mycobacterium leprae (579 aa); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. fadD24 (584 aa); fadD28 (580 aa); etc. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 24h of starvation (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1712302 | 1714053 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1521|fadD25
MSVVESSLPGVLRERASFQPNDKALTFIDYERSWDGVEETLTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGGAHDERTVSVFADTAPAIVLTASSVVDNVVEYVQPQPGQNAPAVIEVDRLDLDARPSSGSRSAAHGHPDILYLQYTSGSTRTPAGVMVSNKNLFANFEQIMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAGIPAVLTSPIGFLQRPARWIQMLASNTLAFTAAPNFAFDLASRKTKDEDMEGLDLGGVHGILNGSERVQPVTLKRFIDRFAPFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDPQKLPDGQAERTESDGGTPLVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIGYWQKPEATERTFSATIVNPSEGTPAGPWLRTGDSGFLSEGELFIMGRIKDLLIVYGRNHSPDDIEATIQTISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKSHGLSVADLVLVSPGSIPITTSGKIRRAQCVELYRQDEFTRLDA
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Trivedi OA et al. [2004]. Enzymic activation and transfer of fatty acids as acyl-adenylates in mycobacteria. Function Product
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
