Gene Rv1537 (dinB1)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in DNA metabolism [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}N]. |
| Product | Probable DNA polymerase IV DinX (pol IV 1) (DNA nucleotidyltransferase (DNA-directed)) |
| Comments | Rv1537, (MTCY48.28c, MT1589), len: 463 aa. Probable dinX (alternate gene name: dinB1), DNA polymerase IV. Similar to umuC, mucB, samb, and impb (UV protection and mutation) e.g. IMPB_SALTY|P18642 impb protein from Salmonella typhimurium (424 aa), FASTA scores: opt: 386, E(): 1.7e-17, (27.5% identity in 415 aa overlap); etc. Also similar to Mycobacterium tuberculosis Rv3056|dinP. Belongs to the DNA polymerase type-Y family. |
| Functional category | Information pathways |
| Proteomics | Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1739856 | 1741247 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1537|dinX
VLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGGRGVVAGASYEARAYGARSAMPMHQARRLIGVTAVVLPPRGVVYGIASRRVFDTVRGLVPVVEQLSFDEAFAEPPQLAGAVAEDVETFCERLRRRVRDETGLIASVGAGSGKQIAKIASGLAKPDGIRVVRHAEEQALLSGLPVRRLWGIGPVAEEKLHRLGIETIGQLAALSDAEAANILGATIGPALHRLARGIDDRPVVERAEAKQISAESTFAVDLTTMEQLHEAIDSIAEHAHQRLLRDGRGARTITVKLKKSDMSTLTRSATMPYPTTDAGALFTVARRLLPDPLQIGPIRLLGVGFSGLSDIRQESLFADSDLTQETAAAHYVETPGAVVPAAHDATMWRVGDDVAHPELGHGWVQGAGHGVVTVRFETRGSGPGSARTFPVDTGDISNASPLDSLDWPDYIGQLSVEGSAGASAPTVDDVGDR
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
