Gene Rv1662
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Potentially involved in some intermediate steps for the synthesis of a polyketide molecule which may be involved in secondary metabolism |
| Product | Probable polyketide synthase Pks8 |
| Comments | Rv1662, (MTCY275.01-MTCY06H11.27), len: 1602 aa. Probable pks8, polyketide synthase, similar to many polyketide synthases e.g. ERY2_SACER|Q03132 erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 3319, E(): 0, (45.8% identity in 1619 aa overlap). Also similar to other Mycobacterium tuberculosis probable polyketide synthases e.g. pks7 and pks12. Contains PS00606 Beta-ketoacyl synthases active site and PS01162 Quinone oxidoreductase/zeta-crystallin signature. Note that the similarity extends into the downstream ORF Rv1663 (MTCY275.02), and this could be accounted for by a frameshift, although the sequence has been checked and no discrepancy was found. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1881704 | 1886512 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1662|pks8
MSGTTTHVDYLKRLTADLRRTRRRLSDLEAKLSEPVAVVGMGCRYPGGVDSPETLWELVAQGRDAVSDFPADRGWDVDGLFDPDPDACGKMYTRRGTFLEHAGDFDAGFFGIGPSEALAMDPQQRLLLEVSWEALERTGIDPTKLRGSATGVFAGVIHAGYGGQLSGELEGYGLTGSTLSVASGRVAYVLGLEGPAVSVDTACSSSLVALHLAVQSLRSGECDLALAGGVTVMATPAAFVEFSRQRALARDGRCKVYAGAADGTAWSEGAGVLVVERLVDARRLGHPVLALVRGSAVNQDGASNGLTAPNGPSQQRVIRAALASARLRAVEVDVVEGHGTGTMLGDPIEAQALLATYGQDRVEPLWLGSIKSNIGHTSAAAGVAGVIKMVQAMRHGVMPKTLHVDVPTPHVDWSVGAVSLLTQPRAWSVHGRPRRAGVSSFGISGTNAHVILEQAPVVESVVPEVASPTAASAVPWVLSARSEQALAGQAQRLLAFVAANPDLDPIDVGWSLVKTRAMFEHRAVVVGADRGALLAGLAALAAGESGAGVAVGRARSVGKTVFVFPGQGAQWVGMGAQLYAELPLFALAFDAVAEELDRHLRLPLRNVLWEGDEALLTSTEFAQPALFAIEVALATLLQHWGISPDFLIGHSVGEIAAAHLAGVLSLTDAAGLVAARGRLMAELPAGGVMVVVAASEEEVLPVLVDGANLAAVNAPHSVVVSGCEAAVSDIADHFARRGRRVHRLAVSHAFHSLLMEPMLAEFTRIAAGISVSKPRIPLVSNVTGQMAGAGYGDGQYWVEHARRPVRFAEGVQLLNAVGATRFVEVGPGGGLTALVEQSLPLGEALSVAMMRREHPEVSSVLGAVATLFTAGAQMDWPAVFGSPGRRIELPTYAFQRQRYWLPPTSAGSADISGVGLLAARHGLLGAVVEQPDSDVVVLTGRLSVGEQRWLADHVIAGVVLLAGAAFVELALRAADQVDCGVVEELTVVTPLVLPTVGGVQLQVVVGVGEMGQRPVSIYSRNAESDSGWVLHARGVLGAKAVAPAADLSVWPPLGAAPVDVDGAYQRFAELGYEYGRAFQGLTAMWRRESELFADVAVPDDVDVTLSGFGIHPLVLDAALHAMGMVGEQAATMLPFSWQGVSLHAAGASRVRARIAPAGDGTVSVELADQAGLPVLSVQALVMRSVSSQLLSAAVAAADAAGRGLLEVAWLPVELAHNDISADLVVWELESFQDGVGPVYSATHRVLVALQSWLAQERAGRLVVLTQGSVGQDATNLAGAAVWGLVRSAQAEHPGRVMLVDSDGSMDVGDVIGCGEEQLMIRNGTAYAARLAQLRPQPILQLPDTNSGWRLVAGGAGALEDLTLASCPAKELAPGQVRIEVRALGVNFRDVLVALGIYPGAAELGAEGAGVVTEVGPGVTGLAVGDPVMGLLGVAGSEAVVDARLVVKLPNRWPLTDAAGVPVVFLTAYYALRVLAQVQPGESVLVHAAAGGVGMAAVQLARLWGLEVFATASRGKWDTLHTMGCDNTHVADSRTLAFEETFWLTTEGRGVDVVLNSLAGEFTDASLRLLPRGGRFIEMGKTEFGTPRSLPRTILGWPTGLST
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
