Gene Rv1688
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in base excision repair. |
| Product | Possible 3-methyladenine DNA glycosylase Mpg |
| Comments | Rv1688, (MTCI125.10), len: 203 aa. Possible mpg, 3-methyladenine DNA glycosylase (see citation below), similar to several eukaryotic 3-methylpurine DNA glycosylases and 3-methyladenine DNA glycosylases e.g. Q39147|X76169 3-methyladenine glycosylase from Arabidobsis thaliana (254 aa), FASTA scores: opt: 297, E(): 8.3e-15, (31.8% identity in 198 aa overlap) and P29372|3MG_HUMAN dna-3-methyladenine glycosidase (298 aa), FASTA scores: opt: 220, E(): 7.2e-05, (36.4% identity in 184 aa overlap). Belongs to the mpg family of DNA glycosylases. |
| Functional category | Information pathways |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1912979 | 1913590 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1688|mpg
MNAEELAIDPVAAAHRLLGATIAGRGVRAMVVEVEAYGGVPDGPWPDAAAHSYRGRNGRNDVMFGPPGRLYTYRSHGIHVCANVACGPDGTAAAVLLRAAAIEDGAELATSRRGQTVRAVALARGPGNLCAALGITMADNGIDLFDPSSPVRLRLNDTHRARSGPRVGVSQAADRPWRLWLTGRPEVSAYRRSSRAPARGASD
Bibliography
- Mizrahi V et al. [1998]. DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence? Secondary Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
