Gene Rv1705c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | PPE family protein PPE22 |
| Comments | Rv1705c, (MTCI125.27c), len: 385 aa. PPE22, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, similar to many e.g. YX23_MYCTU|Q10813 hypothetical 41.1 kDa protein cy274.2 3 (404 aa), fasta scores: opt: 819, E(): 0, (46.2% identity in 413 aa overlap). |
| Functional category | Pe/ppe |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1931497 | 1932654 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1705c|PPE22
MDFGALPPEVNSGRMYCGPGSAPMVAAASAWNGLAAELSVAAVGYERVITTLQTEEWLGPASTLMVEAVAPYVAWMRATAIQAEQAASQARAAAAAYETAFAAIVPPPLIAANRARLTSLVTHNVFGQNTASIAATEAQYAEMWAQDAMAMYGYAGSSATATKVTPFAPPPNTTSPSAAATQLSAVAKAAGTSAGAAQSAIAELIAHLPNTLLGLTSPLSSALTAAATPGWLEWFINWYLPISQLFYNTVGLPYFAIGIGNSLITSWRALGWIGPEAAEAAAAAPAAVGAAVGGTGPVSAGLGNAATIGKLSLPPNWAGASPSLAPTVGSASAPLVSDIVEQPEAGAAGNLLGGMPLAGSGTGTGGAGPRYGFRVTVMSRPPFAG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
