Gene Rv1750c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but supposed involvement in lipid degradation. |
| Product | Possible fatty-acid-CoA ligase FadD1 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) |
| Comments | Rv1750c, (MTCY28.13c, MTCY04C12.34), len: 532 aa. Possible fadD1, fatty-acid-CoA synthetase, similar in part to others e.g. O35488|VLCS_MOUSE very-long-chain acyl-CoA synthetase from Mus musculus (620 aa); NP_113924.1|NM_031736 solute carrier family 27 (fatty acid transporter) member 2 from Rattus norvegicus (620 aa); NP_459076.1|NC_003197 crotonobetaine/carnitine-CoA ligase from Salmonella typhimurium (517 aa); CAIC_ECOLI|P31552 probable crotonobetaine/carnitine-CoA ligase from Escherichia coli (522 aa), FASTA scores: opt: 448, E(): 1.9e-21, (25.1% identity in 502 aa overlap); etc. Also highly similar to fadD17|Rv3506|MTV023.13 probable fatty-acid-CoA ligase from Mycobacterium tuberculosis (502 aa); and similar to others from Mycobacterium tuberculosis e.g. fadD6|MTCI364.18|Rv1206|O05307 probable fatty-acid-CoA ligase (597 aa), FASTA score: (28.3% identity in 519 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1977969 | 1979567 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1750c|fadD1
MTDTIQSLLRQHVSDPTIAVKYGGLQWTWSQYLAESAARAAALITIADPQRPTHIGSLLGNTPEMLAQLAAAGLGGYVLCGLNTTRRGDALAADVRRADCQIVVTDADHRALLDGLDLAGARILDTSTPRWAELVAGDGAFVPYREVDTMDPFMMIFTSGTSGNPKAVPVSHLMATFAGRSLTERFGLTEQDTCYVSMPLFHSNAVVAGWAPAVVSGAAIAPATFSATGFLDDVRRYHATYMNYVGKPLAYILATPERDDDADNPLRVAFGNEANDKDIEEFSRRFGVQVEDGFGSTENAVIVIREPGTPPGSIGRGAHGVAVYNGETVTECAVARFDAHGALTNADEAIGELVNTTGSGFFTGYYNDPEANAERMRHGMYWSGDLAYRDSEGWIYLAGRTADWMRVDGENLTAAPIERILLRYKAINRVAVYAVPDEYVGDQVMAALVLRAGDTFDPDAFEAFLDAQPDLSTKARPRYIRIAADLPSTATHKVLKRQLIDEGTAVGKADTLWVREPRGSAYHHASGPAKAI
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
