Gene Rv1866
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but supposed involvement in lipid degradation. |
| Product | Conserved protein |
| Comments | Rv1866, (MTCY359.07c), len: 778 aa. Conserved protein, N-terminal region similar to fatty acyl-CoA racemases e.g. Rv0855, Rv1143, and C-terminal region (from aa 370) similar to L-carnitine dehydratases, racemases, and Rv3272|MTCY71.12 Mycobacterium tuberculosis (394 aa), FASTA score: opt: 472, E(): 2.1e-21, (29.9% identity in 388 aa overlap). Also similar to P31572|CAIB_ECOLI L-carnitine dehydratase (405 aa), FASTA score: opt: 306, E(): 2.1e-11, (23.3% identity in 424 aa overlap). |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2113140 | 2115476 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1866|Rv1866
MVTRLLADLGADVLKVEPPGGSPGRHVRPTLAGTSIGFAMHNANKRSAVLNPLDESDRRRFLDLAASADIVVDCGLPGQAAAYGASCAELADRYRHLVALSITDFGAAGPRSSWRATDPVLYAMSGALSRSGPTAGTPVLPPDGIASATAAVQAAWAVLVAYFNRLRCGTGDYIDFSRFDAVVMALDPPFGAHGQVAAGIRSTGRWRGRPKNQDAYPIYPCRDGYVRFCVMAPRQWRGLRRWLGEPEDFQDPKYDVIGARLAAWPQISVLVAKLCAEKTMKELVAAGQALGVPITAVLTPSRILASEHFQAVGAITDAELVPGVRTGVPTGYFVVDGKRAGFRTPAPAAGQDEPRWLADPAPVPPPSGRVGGYPFEGLRILDLGIIVAGGELSRLFGDLGAEVIKVESADHPDGLRQTRVGDAMSESFAWTHRNHLALGLDLRNSEGKAIFGRLVAESDAVFANFKPGTLTSLGFSYDVLHAFNPRIVLAGSSAFGNRGPWSTRMGYGPLVRAATGVTRVWTSDEAQPDNSRHPFYDATTIFPDHVVGRVGALLALAALIHRDRTGGGAHVHISQAEVVVNQLDTMFVAEAARATDVAEIHPDTSVHAVYPCAGDDEWCVISIRSDDEWRRATSVFGQPELANDPRFGASRSRVANRSELVAAVSAWTSTRTPVQAAGALQAAGVAAGPMNRPSDILEDPQLIERNLFRDMVHPLIARPLPAETGPAPFRHIPQAPQRPAPLPGQDSVQICRKLLGMTADETERLINERVMFGPAVTA
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
