Gene Rv1981c (nrdF)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the DNA replication pathway. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides, precursors that are necessary for DNA synthesis [catalytic activity: 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H(2)O = ribonucleoside diphosphate + reduced thioredoxin]. |
| Product | Ribonucleoside-diphosphate reductase (beta chain) NrdF1 (ribonucleotide reductase small subunit) (R2F protein) |
| Comments | Rv1981c, (MTCY39.38), len: 322 aa. NrdF1, ribonucleoside-diphosphate reductase, beta chain (see citation below), highly similar to others e.g. RIR4_SALTY|P17424 ribonucleoside-diphosphate reductase (319 aa), FASTA scores: opt: 1402, E(): 0, (66.0% identity in 315 aa overlap); etc. Also similar to Rv3048c|MTV012.63c from Mycobacterium tuberculosis. Contains PS00368 Ribonucleotide reductase small subunit signature. Belongs to the ribonucleoside diphosphate reductase small chain family. Cofactor: binds 2 iron ions (by similarity). Note that previously known as nrdF. |
| Functional category | Information pathways |
| Proteomics | Identified by mass spectrometry in the culture filtrate of M. tuberculosis H37Rv but not the membrane protein fraction or whole cell lysates (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Growth of M. tuberculosis H37Rv nrdF1|Rv1981c mutant is comparable to wild-type in vitro, in vitro in the presence of various drugs and stresses, and in vivo in B6D2/F1 mouse lungs (See Mowa et al., 2009). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2224220 | 2225188 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1981c|nrdF1
MTGKLVERVHAINWNRLLDAKDLQVWERLTGNFWLPEKIPLSNDLASWQTLSSTEQQTTIRVFTGLTLLDTAQATVGAVAMIDDAVTPHEEAVLTNMAFMESVHAKSYSSIFSTLCSTKQIDDAFDWSEQNPYLQRKAQIIVDYYRGDDALKRKASSVMLESFLFYSGFYLPMYWSSRGKLTNTADLIRLIIRDEAVHGYYIGYKCQRGLADLTDAERADHREYTCELLHTLYANEIDYAHDLYDELGWTDDVLPYMRYNANKALANLGYQPAFDRDTCQVNPAVRAALDPGAGENHDFFSGSGSSYVMGTHQPTTDTDWDF
Bibliography
- Yang F et al. [1997]. Characterization of two genes encoding the Mycobacterium tuberculosis ribonucleotide reductase small subunit. Product Biochemistry
- Mowa MB, Warner DF, Kaplan G, Kana BD and Mizrahi V [2009]. Function and regulation of class I ribonucleotide reductase-encoding genes in mycobacteria. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
