Gene Rv2062c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Required for cobalt insertion. |
| Product | Cobalamin biosynthesis protein CobN |
| Comments | Rv2062c, (MTCY49.01c, MTV019.03), len: 1194 aa. cobN, cobalamin biosynthesis protein, similar to many. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2317169 | 2320753 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2062c|cobN
VPEPTVLLLSTSDTDLISARSSGKNYRWANPSRLSDLELTDLLAEASIVVIRILGGYRAWQSGIDTVIAGGVPAVLVSGEQAADAELTDRSTVAAGTALQAHIYLAHGGVDNLRELHAFLCDTVLMTGFGFTPPVATPTWGVLERPDAGKTGPTIAVLYYRAQHLAGNTGYVEALCRAIEDAGGRPLPLYCASLRTAEPRLLERLGGADAMVVTVLAAGGVKPAAASAGGDDDSWNVEHLAALDIPILQGLCLTSPRDQWCANDDGLSPLDVASQVAVPEFDGRIITVPFSFKEIDDDGLISYVADPERCARVAGLAVRHARLRQVAPADKRVALVFSAYPTKHARIGNAVGLDTPASAVALLQAMRQRGYRVGDLPGVESNDGDALIHALIECGGHDPDWLTEGQLAGNPIRVSAKEYRDWFATLPAELTDVVTAYWGPPPGELFVDRSHDPDGEIVIAALRAGNLVLMVQPPRGFGENPVAIYHDPDLPPSHHYLAAYRWLDTGFSNGFGAHAVVHLGKHGNLEWLPGKTLGMSASCGPDAALGDLPLIYPFLVNDPGEGTQAKRRAHAVLVDHLIPPMARAETYGDIARLEQLLDEHASVAALDPGKLPAIRQQIWTLIRAAKMDHDLGLTERPEEDSFDDMLLHVDGWLCEIKDVQIRDGLHILGQNPTGEQELDLVLAILRARQLFGGAHAIPGLRQALGLAEDGTDERATVDQTEAKARELVAALQATGWDPSAADRLTGNADAAAVLRFAATEVIPRLAGTATEIEQVLRALDGRFIPAGPSGSPLRGLVNVLPTGRNFYSVDPKAVPSRLAWEAGVALADSLLARYRDEHGRWPRSVGLSVWGTSAMRTAGDDIAEVLALLGVRPVWDDASRRVIDLAPMQPAELGRPRIDVTVRISGFFRDAFPHVVTMLDDAVRLVADLDEAAEDNYVRAHAQADLAHHGDQRRATTRIFGSKPGTYGAGLLQLIDSRSWRDDADLAQVYTAWGGFAYGRDLDGREAIDDMNRQYRRIAVAAKNTDTREHDIADSDDYFQYHGGMVATVRALTGQAPAAYIGDNTRPDAIRTRTLSEETTRVFRARVVNPRWMAAMRRHGYKGAFEMAATVDYLFGYDATAGVMADWMYEQLTQRYVLDAQNRTFMTESNPWALHGMAERLLEAAGRGLWAQPAPETLDGLRQVLLETEGDLEA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
