Gene Rv2181
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Mannosyltansferase responsible for the addition of alpha(1->2) branches to the mannan core in the biosynthesis of lipomannan (LM) and lipoarabinomannan (lam) |
| Product | Alpha(1->2)mannosyltransferase |
| Comments | Rv2181, (MTV021.14), len: 427 aa. Alpha(1->2)mannosyltransferase (See Kaur et al., 2006). Probable integral membrane protein, similar to others in Mycobacterium tuberculosis e.g. Rv1159 (MTCI65.26, 431 aa). Start uncertain. FASTA scores: Z95584|MTCI65_26 (431 aa) opt: 428, E(): 8e-22; 31.2% identity in 407 aa overlap. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2443302 | 2444585 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2181|Rv2181
MSAWRAPEVGSRLGRRVLWCLLWLLAGVALGYVAWRLFGHTPYRIDIDIYQMGARAWLDGRPLYGGGVLFHTPIGLNLPFTYPPLAAVLFSPFAWLQMPAASVAITVLTLVLLIASTAIVLTGLDAWPTSRLVPAPARLRRLWLAVLIVAPATIWLEPISSNFAFGQINVVLMTLVIVDCFPRRTPWPRGLMLGLGIALKLTPAVFLLYFLLRRDGRAALTALASFAVATLLGFVLAWRDSWEYWTHTLHHTDRIGAAALNTDQNIAGALARLTIGDDERFALWVAGSLLVLAATIWAMRRVLRAGEPTLAVICVALFGLVVSPVSWSHHWVWMLPAVLVIGLLGWRRRNVALAMLSLAGVVLMRWTPIDLLPQHRETTAVWWRQLAGMSYVWWALAVIVVAGLTVTARMTPQRSLTRGLTPAPTAS
Bibliography
- Liu J et al. [2003]. Three monophyletic superfamilies account for the majority of the known glycosyltransferases. Homology
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kaur D et al. [2006]. Biosynthesis of mycobacterial lipoarabinomannan: role of a branching mannosyltransferase. Product Function
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
