Gene Rv2207
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in cobalamin biosynthesis |
| Product | Probable nicotinate-nucleotide-dimethylbenzimidazol phosphoribosyltransferase CobT |
| Comments | Rv2207, (MTCY190.18), len: 361 aa. Probable cobT, phosphoribosyltransferase, similar to many e.g. SW:COBT_ECOLI P36562 nicotinate-nucleotide--dimethylbenzimidazol phosphoribosyltransferase (34.6% identity in 341 aa overlap) |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2471411 | 2472496 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2207|cobT
MIGFAPVSTPDAAAEAAARARQDSLTKPRGALGSLEDLSVWVASCQQRCPPRQFERARVVVFAGDHGVARSGVSAYPPEVTAQMVANIDAGGAAINALADVAGATVRVADLAVDADPLSERIGAHKVRRGSGNIATEDALTNDETAAAITAGQQIADEEVDAGADLLIAGDMGIGNTTAAAVLVAALTDAEPVAVVGFGTGIDDAGWARKTAAVRDALFRVRPVLPDPVGLLRCAGGADLAAIAGFCAQAAVRRTPLLLDGVAVTAAALVAERLAPGAHRWWQAGHRSSEPGHGLALAALGLDPIVDLHMRLGEGTGAAVALMVLRAAVAALSSMATFTEAGVSTRSVDGVDRTAPPAVSP
Bibliography
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
