Gene Rv2221c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Regulatory protein involved in the regulation of glutamine synthetase activity. Adenylylation and deadenylylation of glutamine synthetase. Possibly regulates GLNB|Rv2919c [catalytic activity: ATP + [L-glutamate:ammonia ligase (ADP-forming)] = pyrophosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]]. |
| Product | Glutamate-ammonia-ligase adenylyltransferase GlnE (glutamine-synthetase adenylyltransferase) |
| Comments | Rv2221c, (MTCY190.32c, MTCY427.02c), len: 994 aa. glnE, glutamate-ammonia-ligase adenylyltransferase (see citations below), similar to others e.g. GLNE_ECOLI|P30870 glutamate-ammonia-ligase adenylyltransferase from Escherichia coli, FASTA score: (24.4% identity in 721 aa overlap); GLNE_HAEIN|P44419 Glutamate-ammonia-ligase adenylyltransferase from Haemophilus influenzae (981 aa), FASTA score: (28.1% identity in 199 aa overlap); etc. Note that initiation codon uncertain. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2489369 | 2492353 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2221c|glnE
VVVTKLATQRPKLPSVGRLGLVDPPAGERLAQLGWDRHEDQAHVDLLWSLSRAPDADAALRALIRLSENPDTGWDELNAALLRERSLRGRLFSVLGSSLALGDHLVAHPQSWKLLRGKVTLPSHDQLQRSFVECVEESEGMPGSLVHRLRTQYRDYVLMLAALDLAATVEDEPVLPFTVVAARLADAADAALAAALRVAEASVCGEHPPPRLAVIAMGKCGARELNYVSDVDVIFVAERSDPRNARVASEMMRVASAAFFEVDAALRPEGRNGELVRTLESHIAYYQRWAKTWEFQALLKARPVVGDAELGERYLTALMPMVWRACEREDFVVEVQAMRRRVEQLVPADVRGRELKLGSGGLRDVEFAVQLLQLVHARSDESLRVASTVDALAALGEGGYIGREDAANMTASYEFLRLLEHRLQLQRLKRTHLLPDPEDEEAVRWLARAAHIRPDGRNDAAGVLREELKKQNVRVSKLHTKLFYQPLLESIGPTGLEIAHGMTLEAAGRRLAALGYEGPQTALKHMSALVNQSGRRGRVQSVLLPRLLDWMSYAPDPDGGLLAYRRLSEALATESWYLATLRDKPAVAKRLMHVLGTSAYVPDLLMRAPRVIQQYEDGPAGPKLLETEPAAVARALIASASRYPDPERAIAGARTLRRRELARIGSADLLGLLEVTEVCRALTSVWVAVLQAALDVMIRASLPDDDRAPAAIAVIGMGRLGGAELGYGSDADVMFVCEPATGVDDARAVKWSTSIAERVRALLGTPSVDPPLELDANLRPEGRNGPLVRTLGSYAAYYEQWAQPWEIQALLRAHAVAGDAELGQRFLRMVDKTRYPPDGVSADSVREIRRIKARIESERLPRGADPNTHTKLGRGGLADIEWTVQLLQLQHAHQVPALHNTSTLQSLDVIAAADLVPAADVELLRQAWLTATRARNALVLVRGKPTDQLPGPGRQLNAVAVAAGWRNDDGGEFLDNYLRVTRRAKAVVRKVFGS
Bibliography
- Parish T, Gordhan BG, McAdam RA, Duncan K, Mizrahi V and Stoker NG [1999]. Production of mutants in amino acid biosynthesis genes of Mycobacterium tuberculosis by homologous recombination. Mutant
- Parish T et al. [2000]. glnE is an essential gene in Mycobacterium tuberculosis. Mutant
- Parish T et al. [2001]. Use of the mycobacteriophage L5 excisionase in Mycobacterium tuberculosis to demonstrate gene essentiality. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Hotter GS et al. [2008]. Independent transcription of glutamine synthetase (glnA2) and glutamine synthetase adenylyltransferase (glnE) in Mycobacterium bovis and Mycobacterium tuberculosis. Operon
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
