Gene Rv2300c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved protein |
| Comments | Rv2300c, (MTCY339.09), len: 310 aa (start uncertain). Conserved protein, similar to others e.g. Q9RXY2|DR0172 conserved hypothetical protein from Deinococcus radiodurans (271 aa), FASTA scores: opt: 306, E(): 1.3e-12, (34.6% identity in 229 aa overlap); Q9HZH1|PA3037 hypothetical protein from Pseudomonas aeruginosa (288 aa), FASTA scores: opt: 248, E(): 7.9e-09, (31.5% identity in 238 aa overlap); Q9PDL8|XF1361 hypothetical protein from Xylella fastidiosa (279 aa), FASTA scores: opt: 236, E(): 4.6e-08, (29.7% identity in 249 aa overlap); U70053|XCU70053_3 GumP protein from Xanthomonas campestris (282 aa), FASTA scores: opt: 222, E(): 3.7e-07, (30.1% identity in 248 aa overlap); etc. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2572076 | 2573008 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2300c|Rv2300c
MVATRGRPCPTNFSRPQRPRVAGNGTKSQRCRGRLTTSMLGVAPEAKGPPVKVHHLNCGTMNAFGIALLCHVLLVETDDGLVLVDTGFGIQDCLDPGRVGLFRHVLRPAFLQAETAARQIEQLGYRTSDVRHIVLTHFDFDHIGGIADFPEAHLHVTAAEARGAIHAPSLRERLRYRRGQWAHGPKLVEHGPDGEPWRGFASAKPLDSIGTGVVLVPMPGHTRGHAAVAVDAGHRWVLHCGDAFYHRGTLDGRFRVPFVMRAEEKLLSYNRNQLRDNQARIVELHRRHDPDLLIVCAHDPDLYQLARDTA
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
