Gene Rv2329c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate. |
| Product | Probable nitrite extrusion protein 1 NarK1 (nitrite facilitator 1) |
| Comments | Rv2329c, (MTCY3G12.05), len: 515 aa. Probable narK1, nitrite extrusion protein, possibly member of major facilitator superfamily (MFS). Equivalent to O32974|MLCB22.41c|nark|ML0844 putative nitrite extrusion protein from Mycobacterium leprae (517 aa), FASTA scores: opt: 2224, E(): 1.9e-129, (69.3% identity in 488 aa overlap). Also highly similar to others e.g. P94933 nitrite extrusion protein from Mycobacterium fortuitum (471 aa), FASTA scores: opt: 1969, E(): 8.6e-114, (62.1% identity in 459 aa overlap); P37758|NARU_ECOLI nitrite extrusion protein 2 from Escherichia coli strain K12 (462 aa), FASTA scores: opt: 792, E(): 2.3e-41, (36.95% identity in 476 aa overlap); P10903|NARK_ECOLI nitrite extrusion protein (nitrite facilitator 1) from Escherichia coli strain K12 (463 aa), FASTA scores: opt: 784, E(): 7e-41, (35.3% identity in 468 aa overlap); etc. Also similar to RV0261c|Z86089|MTCY6A4_5 from Mycobacterium tuberculosis (469 aa), FASTA scores: opt: 2000, E(): 1.1e-115, (62.6% identity in 470 aa overlap). Belongs to the nark/NASA family of transporters. |
| Functional category | Cell wall and cell processes |
| Transcriptomics | DNA microarrays show higher level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2601914 | 2603461 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2329c|narK1
MEQHTLLQREESPRSPAAPSLRRLGGSRHITHWDPEDLGAWEAGNKGIARRNLLWSVVTVHLGYSVWTLWPVLELLMPQDVYGFSTSDKFLLGTIATLFGAFLRMPYALASAIFGGRNWATFSAIVLLIPAIGTTVLLTHPGLPLWPYLVCAALTGLGGGNFASSMSNANAFYPHRLKGSALGIAGGVGNLGVPAIQLVGLLAIATVGERKPYLVCALYVVLVAIAVIGVSLFMNNVEQHRVQVNRLRPIVSAVLSTRDTWLLSLLYLGTFGSFIGFSFVFGQVLQTNFLACGQSPARATLHAVELAFVGPLLAAVARIYGGRLADRVGGSRLTLIVFVAMTLAAGLLISASTLEGRHVGQHRGATMVGYFVCFVALFVLSGLGNGSVYKMIPTIFEACSRSLDLSEAERRDWSRIISGVVIGFVAAFGALGGVGINMALRESYLSTGSGTDAFWIFMMCYAAAAVLTWKVYDRRTVTDMGMLQAALVRQPASTPAELIGPRTQSDRFSGCSISA
Bibliography
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
