Gene Rv2344c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | dGTPase preferentially hydrolyzes dGTP over the other canonical NTPS [catalytic activity: dGTP + H(2)O = deoxyguanosine + triphosphate]. |
| Product | Probable deoxyguanosine triphosphate triphosphohydrolase Dgt (dGTPase) (dGTP triphosphohydrolase) |
| Comments | Rv2344c, (MT2409, MTCY98.13c), len: 431 aa. Probable dgt, deoxyguanosine triphosphate triphosphohydrolase, equivalent to Q9CCG3|DGT|ML0831 putative deoxyguanosine triphosphate triphosphohydrolase from Mycobacterium leprae (429 aa), FASTA scores: opt: 2316, E(): 1.6e-137, (83.85% identity in 421 aa overlap); and O52199|DGTP_MYCSM|AF027507_2 deoxyguanosinetriphosphate triphosphohydrolase from Mycobacterium smegmatis (428 aa), FASTA scores: opt: 1991, E(): 3.4e-117, (73.5% identity in 422 aa overlap). Also highly similar or similar to several deoxyguanosine triphosphate hydrolases e.g. Q9L2E9|SC7A8.09c putative deoxyguanosinetriphosphate triphosphohydrolase from Streptomyces coelicolor (424 aa), FASTA scores: opt: 1216, E(): 1e-68, (51.05% identity in 425 aa overlap); BAB48544|MLL1093 dGTP triphosphohydrolase from Rhizobium loti (Mesorhizobium loti) (404 aa), FASTA scores: opt: 489, E(): 3.1e-23, (33.85% identity in 387 aa overlap); P15723|DGTP_ECOLI|DGT|B0160 from Escherichia coli strain K12 (504 aa), FASTA scores: opt: 173, E(): 0.0022, (31.65% identity in 259 aa overlap); etc. Belongs to the dGTPase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2622457 | 2623752 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2344c|dgt
VSASEHDPYDDFDRQRRVAEAPKTAGLPGTEGQYRSDFARDRARVLHSAALRRLADKTQVVGPREGDTPRTRLTHSLEVAQIGRGMAIGLGCDLDLVELAGLAHDIGHPPYGHNGERALDEVAASHGGFEGNAQNFRILTSLEPKVVDAQGLSAGLNLTRASLDAVTKYPWMRGDGLGSQRRKFGFYDDDRESAVWVRQGAPPERACLEAQVMDWADDVAYSVHDVEDGVVSERIDLRVLAAEEDAAALARLGEREFSRVSADELMAAARRLSRLPVVAAVGKYDATLSASVALKRLTSELVGRFASAAIATTRAAAGPGPLVRFRADLQVPDLVRAEVAVLKILALQFIMSDPRHLETQARQRERIHRVAHRLYSGAPQTLDPVYAAAFNTAADDAARLRVVVDQIASYTEGRLERIDADQLGVSRNALD
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
