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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionHydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of Mycobacterium tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate].
ProductMembrane-associated phospholipase C 1 PlcA (MTP40 antigen)
CommentsRv2351c, (MTP40, MT2416, MTCY98.20c), len: 512 aa. plcA (alternate gene name: mpcA), membrane-associated phospolipase C 1 (MTP40 antigen) (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 1064, E(): 4.3e-55, (39.85% identity in 517 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 562, E(): 1.6e-25, (35.35% identity in 481 aa overlap); Q9RGS8|PLCN|PHLN_BURPS non-hemolytic phospholipase C from Burkholderia pseudomallei (Pseudomonas pseudomallei) (700 aa), FASTA scores: opt: 843, E(): 4.4e-42, (40.5% identity in 531 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P95246|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (512 aa), FASTA scores: opt: 2788, E(): 1.2e-156, (75.5% identity in 514 aa overlap) (alias Q50561|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (521 aa), FASTA scores: opt: 2700, E(): 1.8e-151, (73.8% identity in 515 aa overlap)); Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2643, E(): 4.1e-148, (71.6% identity in 511 aa overlap); etc. Belongs to the bacterial phospholipase C family. Supposed membrane-associated, at the extracellular side.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantDisruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2351c|plcA