Gene Rv2351c (mpcA)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of Mycobacterium tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [catalytic activity: a phosphatidylcholine + H(2)O = 1,2- diacylglycerol + choline phosphate]. |
| Product | Membrane-associated phospholipase C 1 PlcA (MTP40 antigen) |
| Comments | Rv2351c, (MTP40, MT2416, MTCY98.20c), len: 512 aa. plcA (alternate gene name: mpcA), membrane-associated phospolipase C 1 (MTP40 antigen) (see citations below), similar to other precursors of several phospolipases C e.g. P15713|PHLN_PSEAE|PA3319 non-hemolytic phospholipase C precursor from Pseudomonas aeruginosa (692 aa), FASTA scores: opt: 1064, E(): 4.3e-55, (39.85% identity in 517 aa overlap); P06200|PHLC_PSEAE hemolytic phospholipase C precursor from Pseudomonas aeruginosa (730 aa), FASTA scores: opt: 562, E(): 1.6e-25, (35.35% identity in 481 aa overlap); Q9RGS8|PLCN|PHLN_BURPS non-hemolytic phospholipase C from Burkholderia pseudomallei (Pseudomonas pseudomallei) (700 aa), FASTA scores: opt: 843, E(): 4.4e-42, (40.5% identity in 531 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P95246|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (512 aa), FASTA scores: opt: 2788, E(): 1.2e-156, (75.5% identity in 514 aa overlap) (alias Q50561|PLCB|MPCB|Rv2350c|MT2415|MTCY98.19c phospholipase C 2 (521 aa), FASTA scores: opt: 2700, E(): 1.8e-151, (73.8% identity in 515 aa overlap)); Q9XB13|PLCD|Rv1755c|MT1799|MTCY28.21C phospholipase C 4 (514 aa), FASTA scores: opt: 2643, E(): 4.1e-148, (71.6% identity in 511 aa overlap); etc. Belongs to the bacterial phospholipase C family. Supposed membrane-associated, at the extracellular side. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2630537 | 2632075 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2351c|plcA
MSRREFLTKLTGAGAAAFLMDWAAPVIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSSTNGFNAASPAFQQMGWNPMTQALDPAGVTIPFRLDTTRGPFLDGECVNDPEHQWVGMHLAWNGGANDNWLPAQATTRAGPYVPLTMGYYTRQDIPIHYLLADTFTICDGYHCSLLTGTLPNRLYWLSANIDPAGTDGGPQLVEPGFLPLQQFSWRIMPENLEDAGVSWKVYQNKGLGRFINTPISNNGLVQAFRQAADPRSNLARYGIAPTYPGDFAADVRANRLPKVSWLVPNILQSEHPALPVALGAVSMVTALRILLSNPAVWEKTALIVSYDENGGFFDHVTPPTAPPGTPGEFVTVPNIDAVPGSGGIRGPLGLGFRVPCIVISPYSRGPLMVSDTFDHTSQLKLIRARFGVPVPNMTAWRDGVVGDMTSAFNFATPPNSTRPNLSHPLLGALPKLPQCIPNVVLGTTDGALPSIPYRVPYPQVMPTQETTPVRGTPSGLCS
Bibliography
- Parra CA et al. [1991]. Isolation, characterization, and molecular cloning of a specific Mycobacterium tuberculosis antigen gene: identification of a species-specific sequence. Sequence
- Johansen KA et al. [1996]. Biochemical and molecular analysis of phospholipase C and phospholipase D activity in mycobacteria. Biochemistry
- Miller BH et al. [2000]. Evaluation of Mycobacterium tuberculosis genes involved in resistance to killing by human macrophages. Secondary Product
- Matsui T et al. [2000]. Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity. Product Regulation
- Parish T et al. [2000]. Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement. Mutant
- Vera-Cabrera L et al. [2001]. Phospholipase region of Mycobacterium tuberculosis is a preferential locus for IS6110 transposition. Sequence Secondary
- Raynaud C, Guilhot C, Rauzier J, Bordat Y, Pelicic V, Manganelli R, Smith I, Gicquel B and Jackson M [2002]. Phospholipases C are involved in the virulence of Mycobacterium tuberculosis. Mutant Function
- Stonehouse MJ et al. [2002]. A novel class of microbial phosphocholine-specific phospholipases C. Homolog Product Biochemistry
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
