Gene Rv2352c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | PPE family protein PPE38 |
| Comments | Rv2352c, (MTCY98.21c), len: 391 aa. PPE38, Member of Mycobacterium tuberculosis PPE_family, highly similar to many e.g. Q10778|MTCY48.17|Y04H_MYCTU (734 aa), FASTA scores: opt: 713, E(): 2.8e-27, (37.7% identity in 430 aa overlap); Q10540|MTCY31.06c, Q11031|MTCY02B10.25c, Q10813|MTCY274.23c, P42611|MTV037.06C, P71868|MTCY03C7.23, P95248|MTCY98.22c, P71869|MTCY03C7.24c, etc. |
| Functional category | Pe/ppe |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2632923 | 2634098 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2352c|PPE38
MILDFSWLPPEINSARIYAGAGSGPLFMAAAAWEGLAADLRASASSFDAVIAGLAAGPWSGPASVAMAGAAAPYVGWLSAAAGQAELSAGQATAAATAFEAALAATVHPAAVTANRVLLGALVATNILGQNTPAIAATEFDYVEMWAQDVGAMVGYHAGAAAVAETLTPFSVPPLDLAGLASQAGAQLTGMATSVSAALSPIAEGAVEGVPAVVAAAQSVAAGLPVDAALQVGQAAAYPASMLIGPMMQLAQMGTTANTAGLAGAEAAGLAAADVPTFAGDIASGTGLGGAGGLGAGMSAELGKARLVGAMSVPPTWEGSVPARMASSAMAGLGAMPAEVPAAGGPMGMMPMPMGMGGAGAGMPAGMMGRGGANPHVVQARPSVVPRVGIG
Bibliography
- Dahl JL et al. [2003]. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice. Regulon
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Proteogenomic analysis of polymorphisms and gene annotation divergences in prokaryotes using a clustered mass spectrometry-friendly database. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
