Gene Rv2359 (furB)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Acts as a global negative controlling element, employing Zn(2+) as a cofactor to bind the operator of the repressed genes. |
| Product | Probable zinc uptake regulation protein Zur |
| Comments | Rv2359, (MTCY27.21c), len: 130 aa. Probable zur, zinc uptake regulation protein, equivalent to FURB|ML0824|Q9CCG6 putative ferric uptake regulatory protein from Mycobacterium leprae (131 aa), FASTA scores: opt: 765, E(): 1.7e-43, (86.9% identity in 130 aa overlap). Also highly similar to ferric uptake regulation proteins e.g. Q9L2H5|SCC121.11 putative metal uptake regulation protein from Streptomyces coelicolor (139 aa), FASTA scores: opt: 547, E(): 3.4e-29, (59.4% identity in 133 aa overlap); P06975|FUR_ECOLI from Escherichia coli (148 aa), FASTA scores: opt: 322, E(): 1.9e-14, (37.9% identity in 132 aa overlap); P45599|FUR_KLEPN ferric uptake regulation protein from Klebsiella pneumoniae (155 aa), FASTA scores: opt: 314, E(): 6.7e-14, (36.35% identity in 132 aa overlap); etc. Belongs to the fur/ZUR family. Note that previously known as furB. |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | Note that in Mycobacterium bovis BCG, Northern blotting analysis and cDNA-total RNA subtractive hybridization strategy reveals increased expression of the corresponding transcript while inside macrophages (see Li et al., 2001). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Growth of M. tuberculosis H37Rv zur|Rv2359 mutant in C57BL/6 mice is similar to wild-type (See Maciag et al., 2007). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2641650 | 2642042 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2359|zur
MSAAGVRSTRQRAAISTLLETLDDFRSAQELHDELRRRGENIGLTTVYRTLQSMASSGLVDTLHTDTGESVYRRCSEHHHHHLVCRSCGSTIEVGDHEVEAWAAEVATKHGFSDVSHTIEIFGTCSDCRS
Bibliography
- Li MS, Monahan IM, Waddell SJ, Mangan JA, Martin SL, Everett MJ and Butcher PD [2001]. cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. Homolog Transcriptome
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Milano A et al. [2004]. The Mycobacterium tuberculosis Rv2358-furB operon is induced by zinc. Regulation
- Canneva F et al. [2005]. Rv2358 and FurB: two transcriptional regulators from Mycobacterium tuberculosis which respond to zinc. Regulation
- Maciag A et al. [2007]. Global analysis of the Mycobacterium tuberculosis Zur (FurB) regulon. Function Mutant Product
- Lucarelli D et al. [2007]. Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis. Function Product Structure
- Jiang H et al. [2008]. Prokaryotic expression and monoclonal antibody preparation of Mycobacterium tuberculosis ferric uptake regulator B. Biochemistry
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
