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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionActs as a co-chaperone. Stimulates, jointly with GRPE, the ATPase activity of DNAK|Rv0350.
ProductProbable chaperone protein DnaJ2
CommentsRv2373c, (MTCY27.07), len: 382 aa. Probable dnaJ2, chaperone protein, equivalent to Q49762|DNJ2_MYCLE|ML0625|B1937_F2_56 chaperone protein from Mycobacterium leprae (378 aa), FASTA scores: opt: 2301, E(): 1.7e-120, (87.5% identity in 382 aa overlap). Also highly similar to other chaperone proteins DNAJ/DNAJ2 e.g. Q9RDD7|DNJ2_STRCO|SCC77.21c from Streptomyces coelicolor (378 aa), FASTA scores: opt: 1456, E(): 1.2e-73, (54.8% identity in 385 aa overlap); O52164|DNJ2_STRAL from Streptomyces albus (379 aa) FASTA scores: opt: 1378, E(): 2.6e-69, (52.2% identity in 385 aa overlap); Q9S5A3|DNAJ_LISMO from Listeria monocytogenes (377 aa), FASTA scores: opt: 1013, E(): 4.6e-49, (41.3% identity in 385 aa overlap); etc. Also similar to Rv0352|MTCY13E10.12 from Mycobacterium tuberculosis. Contains 1 J domain and 1 cr domain. Belongs to the DNAJ family.
Functional categoryVirulence, detoxification, adaptation
ProteomicsIdentified by proteomics at the Statens Serum Institute (Denmark) (see Rosenkrands et al., 2000). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011).
TranscriptomicsmRNA identified by DNA microarray analysis and up-regulated at high temperatures (see Stewart et al., 2002).
MutantDisruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS26528392653987-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2373c|dnaJ2
VARDYYGLLGVSKNASDADIKRAYRKLARELHPDVNPDEAAQAKFKEISVAYEVLSDPDKRRIVDLGGDPLESAAAGGNGFGGFGGLGDVFEAFFGGGFGGGAASRGPIGRVRPGSDSLLRMRLDLEECATGVTKQVTVDTAVLCDRCQGKGTNGDSVPIPCDTCGGRGEVQTVQRSLLGQMLTSRPCPTCRGVGVVIPDPCQQCMGDGRIRARREISVKIPAGVGDGMRVRLAAQGEVGPGGGPAGDLYVEVHEQAHDVFVREGDHLHCTVSVPMVDAALGVTVTVDAILDGLSEITIPPGTQPGSVITLRGRGMPHLRSNTRGDLHVHVEVVVPTRLDHQDIELLRELKGRRDREVAEVRSTHAAAGGLFSRLRETFTGR