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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in transcriptional regulation (repression) of class I heat shock proteins e.g. DNAK-GRPE-DNAJ1 and groels operons). Prevents heat-shock induction of these operons.
ProductProbable heat shock protein transcriptional repressor HrcA
CommentsRv2374c, (MTCY27.06), len: 343 aa. Probable hrcA, heat-inducible transcriptional repressor (see citation below), equivalent to Q9CCN2|HRCA|ML0624 putative heat-inducible transcriptional regulator from Mycobacterium leprae (343 aa), FASTA scores: opt: 1926, E(): 3.9e-107, (89.8% identity in 343 aa overlap). Also highly similar to other heat-inducible transcription repressor proteins e.g. Q9RDD6|HRCA|SCC77.22c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 1227, E(): 1.1e-65, (58.8% identity in 335 aa overlap); O52163|HRCA_STRAL from Streptomyces albus (338 aa), FASTA scores: opt: 1196, E(): 7.7e-64, (56.1% identity in 335 aa overlap); P25499|HRCA_BACSU heat-inducible transcription repressor from Bacillus subtilis (343 aa), FASTA scores: opt: 538, E(): 8.4e-25, (28.9% identity in 325 aa overlap); etc. Almost identical, but conflict at C-terminus, to Q49749|YGRP|B1937_F1_18 putative heat-inducible transcription repressor from Mycobacterium leprae (197 aa) FASTA scores: opt: 1126, E(): 6.9e-60, (91.8% identity in 195 aa overlap). Belongs to the HRCA family.
Functional categoryVirulence, detoxification, adaptation
ProteomicsIdentified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS26540622655093-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2374c|hrcA
MGSADERRFEVLRAIVADFVATQEPIGSKSLVERHNLGVSSATVRNDMAVLEAEGYITQPHTSSGRVPTEKGYREFVDRLEDVKPLSSAERRAIQSFLESGVDLDDVLRRAVRLLAQLTRQVAVVQYPTLSTSTVRHLEVIALTPARLLMVVITDSGRVDQRIVELGDVIDDHQLAQLREILGQALEGKKLSAASVAVADLASQLGGAGGLGDAVGRAATVLLESLVEHTEERLLLGGTANLTRNAADFGGSLRSILEALEEQVVVLRLLAAQQEAGKVTVRIGHETASEQMVGTSMVSTAYGTAHTVYGGMGVVGPTRMDYPGTIASVAAVALYIGDVLGAR