Gene Rv2380c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Probably activates the two lysine residues that are incorporated into mycobactin (lysine ligation). |
| Product | Peptide synthetase MbtE (peptide synthase) |
| Comments | Rv2380c, (MTCY22H8.05), len: 1682 aa. MbtE, peptide synthetase (see citations below), similar in part to several synthases e.g. O07944|SNBDE pristinamycin I synthase 3 and 4 from Streptomyces pristinaespiralis (4848 aa), FASTA scores: opt: 2635, E(): 1.9e-146, (36.8% identity in 1657 aa overlap); O05647|SNBDE virginiamycin S synthetase (fragment) from Streptomyces virginiae (1997 aa) FASTA scores: opt: 2580, E(): 1.6e-143, (40.65% identity in 1163 aa overlap); Q9R9I2|DHBF protein involved in siderophore production from Bacillus subtilis (2378 aa), FASTA scores: opt: 2388, E(): 3.6e-132, (33.9% identity in 1579 aa overlap); O68487|ACMB actinomycin synthetase II from Streptomyces chrysomallus (2611 aa), FASTA scores: opt: 2165, E(): 4.9e-119, (35.0% identity in 1634 aa overlap); etc. Equivalent to AAK46743 from Mycobacterium tuberculosis strain CDC1551 (1787 aa) but shorter 105 aa. Contains PS00455 putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | DNA microarrays indicate repression by iron and IdeR|Rv2711 in M. tuberculosis H37Rv (See Rodriguez et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2662067 | 2667115 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2380c|mbtE
MWFVQMADPSGALLNICVSYRITGDIDLARLRDAVNAVARRHRILRTTYPVGDDGVAQPTVHADLRPGWTQYDLTDLSQRAQRLRLEVLAQREFCAPFELSRDAPLRITVVRTAADEHVLLLVAHHIAWDDGSWRVFFTDLTQAYSRADLGADLGPEHRPSAASGPDTTEADLNYWRAIMADPPEPLELPGPAGTCVPTSWRAARATLRLPADTAARVATMAKNTGCTPYMVLLAAFGALVHRYTHSDDFLVAAPVLNRGAGTEDAIGYFGNTVAMRLRPQSAMSFRELLTATRDIASGAFAHQRINLDRVVRELNPDRRHGAERMTRVSFGFREPDGGGFNPPGIECERYDLRSNITQLPLGFMVEFDRAGVLVEAEHLVEILEPALAKQMLRHFGVLLDNALAAPDNTLSGLALMDERDAARLREVSRGERFDTPVKTLVDLVNEQTTRTPDATAVVYEGQHFTYHDLNEASNRLGHWLIEQGIGSEDRVAVLLDKSPDLIVTALGVVKSGAVYVPVDPSYPQDRLDFILADCDAKLVLRTPVRELAGYRSDDPTDADRIRPLRPDNTAYLIYTSGTTGLPKGVAVPHRPVAEYFVWFKGEYDVDDTDRLLQVASPSFDVSIAEIFGTLACGARMVIPRPGGLTDIGYLTALLRDEGITAMHFVPSLLGLFLSLPGVSQWRTLQRVPIGGEPLPGEVADKFHATFDALLHNFYGPTETVINASRFKVVGPQGTRIVPIGRPKINTTMHLLDDSLQPVPTGVIGEIYIGGTHVAYGYHRRAGLTAERFVADPFNPGSRMYRSGDLARRNADGDIEFVGRADEQVKIRGFRIELGDVAAAIAVDPTVGQAVVVVSDLPRLGKSLVGYVTPAAGGDGPADVGVDLDRIRARVAAALPEYMLPAAYVVLDEIPITAHGKIDRAALPEPQIASDTEFRAPQTATERRLAQLFGELLGRDRVGADDSFFDLGGHSLLATKLVAAVRNAFGVDVGVREIFEFATVTALAGHIDTLDSDSARPRLTRVDHDGPVRLSSSQMRSWFNYRFDGPNAVNNIPFAAALHGPCDTNAFAAAITDVVARHEILRTVYREIGGVPHQIIQPPAEVPVRCAAGSDAAWLRAELNNERGYVFDLETDWPIRAALLSTPEQTVLSLVVHHIAGDHWSAGVLFTDLLTAYRARSTGQRPSWAPLPVQYADYSVWQSALLDDGAGIVGPQRDYWIRQLGGLAGETGLRPDFPRPALLSGAGDAVEFRLGAAIRDKLAAVSRDLGVTEFMLLQAAVAVVLHKAGGGVDVPIGAPVAGRSEANLDQLIGFFINIVVLRNDLRGNPTLREVLQRTRQMALAAYAHQDLPFDQVVEAVNPQRSLSRNPLFDIVVHVREQMPQDHVIDTGPDGDTTLRVLEPTFDAAQADLSVNFFACGDEYRGHVIYRTELYERATAQRFADWLVRVVEAFADRPDQPLREVEMVSAQARRRILDRSNAGAGTARVYLLDDALKPVPVGVVGDVYYGGGPAVGARLARPSETATRFVADPFAAQPGSRLYRNGERGVWKADGQLELLAEIERLPTAQAAPVPAEPADTETERALAAILADVLEVGEVGRYDDFFNLGGDSILATQVAARARDGGIPLTARMVFEHPVLCELAAAVDAKPHVEAEPDDKHHAPMSTSGLSPDELSALTASWDQWP
Bibliography
- Quadri LE et al. [1998]. Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin. Secondary Function
- De Voss JJ et al. [1999]. Iron acquisition and metabolism by mycobacteria. Review
- Rodriguez GM, Voskuil MI, Gold B, Schoolnik GK and Smith I [2002]. ideR, An essential gene in mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Transcriptome
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
