Gene Rv2381c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. |
| Product | Polyketide synthetase MbtD (polyketide synthase) |
| Comments | Rv2381c, (MTCY22H8.04), len: 1004 aa. MbtD, polyketide synthase (see citations below), similar in part to several synthases e.g. Q03132|ERY2_SACER|ERYA erythronolide synthase, modules 3 and 4 from Saccharopolyspora erythraea (Streptomyces erythraeus) (3567 aa), FASTA scores: opt: 971, E(): 1e-46, (29.35% identity in 1043 aa overlap); Q9F829|megaii megalomicin 6-deoxyerythronolide B synthase 2 from Micromonospora megalomicea subsp. nigra (3562 aa), FASTA scores: opt: 787, E(): 2.4e-36, (29.35% identity in 1032 aa overlap); Q9L4W4|NYSB polyketide synthase from Streptomyces noursei (3192 aa), FASTA scores: opt: 761, E(): 6.6e-35, (29.55% identity in 1086 aa overlap); O30764|NIDA1 polyketide synthase modules 1 and 2 from Streptomyces caelestis (4340 aa), FASTA scores: opt: 726, E(): 7.8e-33, (27.3% identity in 1052 aa overlap); etc. Contains PS00012 Phosphopantetheine attachment site. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 and 90 days (See Kruh et al., 2010). |
| Transcriptomics | DNA microarrays indicate repression by iron and IdeR|Rv2711 in M. tuberculosis H37Rv (See Rodriguez et al., 2002). DNA microarrays show increased expression in M. tuberculosis H37Rv in BALB/c mice compared to SCID mice, after 21 days of infection (See Talaat et al., 2004). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2667255 | 2670269 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2381c|mbtD
MAPKQLPDGRVAVLLSAHAEELIGPDARAIADYLERFPATTVTEVARQLRKTRRVRRHRAVLRAADRLELAEGLRALAAGREHPLIARSSLGSAPRQAFVFPGQGGHWPGMGAVAYRELPTYRTATDTCAAAFAAAGVDSPLPYLIAPPGTDERQAFCEIEIEGAQFVHAVALAEVWRSCGVLPDLTVGHSLGEVAAAYLAGSITLSDAVAVVAARANVVGRLPGRYAVAALGIGEQDASALIATTGGWLELSVVNASSTVAVSGERQAVAAIVDTVRSSGHFARGITVGFPVHTSVLESLRDELCEQLPDSEFMEAPVQFIGGTTGDVVAPGTTFGDYWYANLRHTVRFDRAVESAIRCGARAFIEISAHPALLFAIGQNCEGAANLPDGPAVLVGSARRGERFVDALSANIVSAAVADPGYPWGDLGGDPLDGDVDLSGFPNAPMRAVPMWAHPEPLPPVSGLTIAVERWERMVPSTPVAGRHRHLAVLDLGAHRALAQTLCAAIDSHPDTELSAARDAELILVIAPDFEHTDAVRAAGALADLVGAGLLDYPMHIGARCQSVCLVTVGAEQVDAADAVPSAGQAALAAMHRSIGFEHPEQTFSHLDLPSWDLDPVLGVSVITAVLRGFGETALRGSVNGYTLFERTLADAPAVPNWSLDSGVLDDVVVTGGAGAIGMHYARYLAEHGARRIVLLSRRAADQATVAMLRKQHGTVIVSPPCDITDPTQLSAIAAEYGGVGASLIVHAAGSVISGTAPGVTSAAVVDNFAAKVLGLAQMIELWPLRPDVRTLLCSSVMGVWGGHGVVAYSAANRLLDVMAAQLRAQGRHCVAVKWGLWQAPKAGEPARGIADAVTIARVERSGLRQMAPQQAIEASLHEFTVDPLVFAADAARLQMLLDSRQFERYEGPTDPNLTIVDAVRTQLAAVLGIPQAGEVNLQESLFDLGVDSMLALDLRNRLKRSIGATVSLATLMGDITGDGLVAKLEDADERSHTAQKVDISRD
Bibliography
- Quadri LE et al. [1998]. Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin. Secondary Function
- De Voss JJ et al. [1999]. Iron acquisition and metabolism by mycobacteria. Review
- Rodriguez GM, Voskuil MI, Gold B, Schoolnik GK and Smith I [2002]. ideR, An essential gene in mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Transcriptome
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Talaat AM et al. [2004]. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Transcriptome
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
