Gene Rv2383c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. This peptide synthase forms amide bound between the carboxylic acid of salicylate and the alpha-amino group of serine (serine/threonine ligation). |
| Product | Phenyloxazoline synthase MbtB (phenyloxazoline synthetase) |
| Comments | Rv2383c, (MTCY22H8.02), len: 1414 aa. MbtB, phenyloxazoline synthase (see citations below), similar to the N-terminal region of several synthetases e.g. Q9EWP5|SC4C2.17 putative non-ribosomal peptide synthase from Streptomyces coelicolor (2229 aa), FASTA scores: opt: 2878, E(): 4.1e-156, (46.85% identity in 1138 aa overlap); Q9Z399|IRP2 yersiniabactin biosynthetic from Yersinia pestis (2041 aa), FASTA scores: opt: 2297, E(): 5.3e-123, (38.55% identity in 1069 aa overlap); P48633|HMP2_YEREN|IRP2 high-molecular-weight protein 2 (may be involved in the nonribosomal synthesis of small peptides) from Yersinia enterocolitica (2035 aa), FASTA scores: opt: 2275, E(): 9.4e-122, (38.45% identity in 1069 aa overlap); O85739|PCHE|PA4226 dihydroaeruginoic acid synthetase from Pseudomonas aeruginosa (1438 aa) FASTA scores: opt: 2236, E(): 1.2e-119, (38.2% identity in 1330 aa overlap); Q9RFM8|PCHE pyochelin synthetase from Pseudomonas aeruginosa (1438 aa), FASTA scores: opt: 2229, E(): 3e-119, (38.0% identity in 1329 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature, and PS00012 Phosphopantetheine attachment site. Belongs to the ATP-dependent AMP-binding enzyme family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 and 90 days (See Kruh et al., 2010). |
| Transcriptomics | DNA microarrays indicate repression by iron and IdeR|Rv2711 in M. tuberculosis H37Rv (See Rodriguez et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Slow growth mutant by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2671593 | 2675837 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2383c|mbtB
VVHATACSEIIRAEVAELLGVRADALHPGANLVGQGLDSIRMMSLVGRWRRKGIAVDFATLAATPTIEAWSQLVSAGTGVAPTAVAAPGDAGLSQEGEPFPLAPMQHAMWVGRHDHQQLGGVAGHLYVEFDGARVDPDRLRAAATRLALRHPMLRVQFLPDGTQRIPPAAGSRDFPISVADLRHVAPDVVDQRLAGIRDAKSHQQLDGAVFELALTLLPGERTRLHVDLDMQAADAMSYRILLADLAALYDGREPPALGYTYREYRQAIEAEETLPQPVRDADRDWWAQRIPQLPDPPALPTRAGGERDRRRSTRRWHWLDPQTRDALFARARARGITPAMTLAAAFANVLARWSASSRFLLNLPLFSRQALHPDVDLLVGDFTSSLLLDVDLTGARTAAARAQAVQEALRSAAGHSAYPGLSVLRDLSRHRGTQVLAPVVFTSALGLGDLFCPDVTEQFGTPGWIISQGPQVLLDAQVTEFDGGVLVNWDVREGVFAPGVIDAMFTHQVDELLRLAAGDDAWDAPSPSALPAAQRAVRAALNGRTAAPSTEALHDGFFRQAQQQPDAPAVFASSGDLSYAQLRDQASAVAAALRAAGLRVGDTVAVLGPKTGEQVAAVLGILAAGGVYLPIGVDQPRDRAERILATGSVNLALVCGPPCQVRVPVPTLLLADVLAAAPAEFVPGPSDPTALAYVLFTSGSTGEPKGVEVAHDAAMNTVETFIRHFELGAADRWLALATLECDMSVLDIFAALRSGGAIVVVDEAQRRDPDAWARLIDTYEVTALNFMPGWLDMLLEVGGGRLSSLRAVAVGGDWVRPDLARRLQVQAPSARFAGLGGATETAVHATIFEVQDAANLPPDWASVPYGVPFPNNACRVVADSGDDCPDWVAGELWVSGRGIARGYRGRPELTAERFVEHDGRTWYRTGDLARYWHDGTLEFVGRADHRVKISGYRVELGEIEAALQRLPGVHAAAATVLPGGSDVLAAAVCVDDAGVTAESIRQQLADLVPAHMIPRHVTLLDRIPFTDSGKIDRAEVGALLAAEVERSGDRSAPYAAPRTVLQRALRRIVADILGRANDAVGVHDDFFALGGDSVLATQVVAGIRRWLDSPSLMVADMFAARTIAALAQLLTGREANADRLELVAEVYLEIANMTSADVMAALDPIEQPAQPAFKPWVKRFTGTDKPGAVLVFPHAGGAAAAYRWLAKSLVANDVDTFVVQYPQRADRRSHPAADSIEALALELFEAGDWHLTAPLTLFGHCMGAIVAFEFARLAERNGVPVRALWASSGQAPSTVAASGPLPTADRDVLADMVDLGGTDPVLLEDEEFVELLVPAVKADYRALSGYSCPPDVRIRANIHAVGGNRDHRISREMLTSWETHTSGRFTLSHFDGGHFYLNDHLDAVARMVSADVR
Bibliography
- Quadri LE et al. [1998]. Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin. Secondary Function
- De Voss JJ et al. [1999]. Iron acquisition and metabolism by mycobacteria. Review
- De Voss JJ et al. [2000]. The salicylate-derived mycobactin siderophores of Mycobacterium tuberculosis are essential for growth in macrophages. Mutant Function
- Gold B et al. [2001]. The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages. Regulon
- Rodriguez GM, Voskuil MI, Gold B, Schoolnik GK and Smith I [2002]. ideR, An essential gene in mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
