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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins. Possibly plays a role in the conversion of chorismate to salicilate (the starter unit for mycobactin siderophore construction).
ProductIsochorismate synthase MbtI
CommentsRv2386c, (MTCY253.35), len: 450 aa. mbtI, isochorismate synthase (see citations below), similar to Q9X9I8|IRP9 salicylate synthetase from Yersinia enterocolitica (434 aa), FASTA scores: opt: 887, E(): 7.5e-48, (37.45% identity in 422 aa overlap); and similar in C-terminal region to many anthranilate synthases component I e.g. Q9Z4W7|TRPE_STRCO|SCE8.07c from Streptomyces coelicolor (511 aa), FASTA scores: opt: 509, E(): 3e-24, (40.4% identity in 255 aa overlap); P33975|TRPE_HALVO from Halobacterium volcanii (Haloferax volcanii) (523 aa) FASTA scores: opt: 488, E(): 6.2e-23, (34.2% identity in 298 aa overlap); and similar to Q08653|TRPE_THEMA|TM0142 anthranilate synthase component I from Thermotoga maritima (461 aa), FASTA scores: opt: 478, E(): 2.3e-22, (28.4% identity in 440 aa overlap); etc. Could be belong to the anthranilate synthase component I family. Note that previously known as trpE2, an anthranilate synthase component I.
Functional categoryLipid metabolism
ProteomicsIdentified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005).
TranscriptomicsDNA microarrays indicate repression by iron and IdeR|Rv2711 in M. tuberculosis H37Rv (See Rodriguez et al., 2002).
MutantDisruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS26786532680005-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2386c|mbtI
VSELSVATGAVSTASSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQLGGIRALGYSPELVTAVRADGVVITEPLAGTRALGRGPAIDRLARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAVIDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVARQ
      
Bibliography