Gene Rv2388c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in porphyrin biosynthesis. Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX. |
| Product | Probable oxygen-independent coproporphyrinogen III oxidase HemN (coproporphyrinogenase) (coprogen oxidase) |
| Comments | Rv2388c, (MTCY253.33), len: 375 aa. Probable hemN, oxygen-independent coproporphyrinogen III oxidases, highly similar to many putative oxygen-independent coproporphyrinogen III oxidases e.g. Q9RDD2|SCC77.26 from Streptomyces coelicolor (435 aa), FASTA scores: opt: 1358, E(): 1.5e-76, (56.55% identity in 382 aa overlap); BAB51237|MLR4627 from Rhizobium loti (Mesorhizobium loti) (392 aa), FASTA scores: opt: 696, E(): 1.1e-35, (36.8% identity in 383 aa overlap); Q9KUR0|VC0455 from Vibrio cholerae (391 aa), FASTA scores: opt: 691, 2.2e-35, (32.65% identity in 386 aa overlap); P54304|HEMN_BACSU from Bacillus subtilis (366 aa), FASTA scores: opt: 668 , E(): 5.6e-34; (34.9% identity in 327 aa overlap); etc. Equivalent to AAK46752 from Mycobacterium tuberculosis strain CDC1551 (390 aa) but shorter 375 aa. Belongs to the anaerobic coproporphyrinogen III oxidase family. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2682015 | 2683142 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2388c|hemN
MPGQPFGVYLHVPFCLTRCGYCDFNTYTPAQLGGVSPDRWLLALRAELELAAAKLDAPTVHTVYVGGGTPSLLGGERLATLLDMVRDHFVLAPDAEVSTEANPESTWPEFFATIRAAGYTRVSLGMQSVAPRVLATLDRVHSPGRAAAAATEAIAEGFTHVNLDLIYGTPGESDDDLVRSVDAAVQAGVDHVSAYALVVEHGTALARRVRRGELAAPDDDVLAHRYELVDARLSAAGFAWYEVSNWCRPGGECRHNLGYWDGGQWWGAGPGAHGYIGVTRWWNVKHPNTYAEILAGATLPVAGFEQLGADALHTEDVLLKVRLRQGLPLARLGAAERERAEAVLADGLLDYHGDRLVLTGRGRLLADAVVRTLLG
Bibliography
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
