Go to browser
virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionPromotes the resuscitation and growth of dormant, nongrowing cell. Could also stimulates the growth of several other high G+C gram+ organisms, e.g. Mycobacterium avium, Mycobacterium bovis (BCG), Mycobacterium kansasii, Mycobacterium smegmatis.
ProductProbable resuscitation-promoting factor RpfD
CommentsRv2389c, (MTCY253.32), len: 154 aa. Probable rpfD, resuscitation-promoting factor. Possible autocrine and/or paracrine bacterial growth factor or cytokine (see citation below). Similar to others from Mycobacterium tuberculosis e.g. O07747|Rv1884c|MTCY180.34|RPFC probable resuscitation-promoting factor from Mycobacterium tuberculosis (176 aa), FASTA scores: opt: 382, E(): 2.3e-17, (55.45% identity in 101 aa overlap); etc. Also similarity with Q9CBF8|ML2030 hypothetical protein from Mycobacterium leprae (157 aa), FASTA scores: opt: 397, E(): 2.4e-18, (47.95% identity in 121 aa overlap); Q9F2Q2|SCE41.06c putative secreted protein from Streptomyces coelicolor (244 aa), FASTA scores: opt: 341, E(): 1.1e-14, (40.45% identity in 131 aa overlap); and O86308|Z96935|MLRPF_1 RPF protein precursor from Micrococcus luteus (220 aa), FASTA scores: opt: 301, E(): 3.6e-12, (39.4% identity in 132 aa overlap). Contains a secretory signal sequence in N-terminus. Supposed acts at very low concentration. Predicted possible vaccine candidate (See Zvi et al., 2008).
Functional categoryCell wall and cell processes
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Non essential gene by specialized transduction in M. tuberculosis Erdman; growth and persistence of mutant in mice are not attenuated (See Tufariello et al., 2004). M. tuberculosis H37Rv rpfACBD, rpfACBE, rpfACDE, and rpfACBED mutants show no growth defects in broth culture; growth of rpfACBD and rpfACBE mutants in human peripheral blood mononuclear cells is unaffected but growth in B6D2/F1 mice is attenuated (See Kana et al., 2008).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS26832482683712-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2389c|rpfD
MTPGLLTTAGAGRPRDRCARIVCTVFIETAVVATMFVALLGLSTISSKADDIDWDAIAQCESGGNWAANTGNGLYGGLQISQATWDSNGGVGSPAAASPQQQIEVADNIMKTQGPGAWPKCSSCSQGDAPLGSLTHILTFLAAETGGCSGSRDD
      
Bibliography