Gene Rv2398c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the active transport across the membrane of multiple sulfur-containing compounds, including sulfate and thiosulfate (import). Responsible for the translocation of the substrate across the membrane. |
| Product | Probable sulfate-transport integral membrane protein ABC transporter CysW |
| Comments | Rv2398c, (MTCY253.23), len: 272 aa. Probable cysW, sulfate-transport integral membrane protein ABC transporter (see citations below), similar to others e.g. Q9K877|CYSW|BH3129 sulfate ABC transporter (permease) from Bacillus halodurans (287 aa), FASTA scores: opt: 765, E(): 4.1e-40, (43.8% identity in 249 aa overlap); P27370|CYSW_SYNP7 sulfate transport system (permease) protein from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (286 aa), FASTA scores: opt: 757, E(): 1.3e-39, (44.3% identity in 264 aa overlap); Q9I6K9|CYSW|PA0281 sulfate transport protein from Pseudomonas aeruginosa (289 aa), FASTA scores: opt: 753, E(): 2.3e-39, (44.4% identity in 250 aa overlap); P16702|P76534|CYSW_ECOLI sulfate transport system permease from Escherichia coli (291 aa), FASTA scores: opt: 633, E(): 5.7e-32, (38.2% identity in 267 aa overlap); etc. Contains PS00402 Binding-protein-dependent transport systems inner membrane component signature. Similarity with integral membrane components of other binding-protein-dependent transport systems and belongs to the CYSTW subfamily. |
| Functional category | Cell wall and cell processes |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 24h and 96h of starvation (see Betts et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2694981 | 2695799 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2398c|cysW
MTSLPAARYLVRSVALGYVFVLLIVPVALILWRTFEPGFGQFYAWISTPAAISALNLSLLVVAIVVPLNVIFGVTTALVLARNRFRGKGVLQAIIDLPFAVSPVIVGVSLILLWGSAGALGFVEQDLGFKIIFGLPGIVLGSMFVTCPFVVREVEPVLHELGTDQEQAAATLGSGWWQTFWRITLPSIRWGLTYGIVLTVARTLGEYGAVIIVSSNLPGTSQTLTLLVSDRYHRGAEYGAYALSTLLMAVSVVVLIVQMVLDARRARAVSEG
Bibliography
- Braibant M et al. [2000]. The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. Review Secondary
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Wooff E et al. [2002]. Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo. Homolog Secondary Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
