Gene Rv2459
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in a transport system across the membrane (perhaps drug transport): responsible for the translocation of the substrate across the membrane. |
| Product | Probable conserved integral membrane transport protein |
| Comments | Rv2459, (MTV008.15), len: 508 aa. Probable conserved integral membrane transport protein, member of major facilitator superfamily (MFS) possibly involved in drug transport, highly similar to many efflux proteins e.g. Q9RL22|SC5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 788, E(): 1.3e-38, (34.45% identity in 412 aa overlap); Q9I428|PA1316 probable MFS transporter from Pseudomonas aeruginosa (513 aa), FASTA scores: opt: 782, E(): 3.1e-38, (32.75% identity in 519 aa overlap); P39886|TCMA_STRGA tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 752, E(): 1.8e-36, (31.7% identity in 511 aa overlap); etc. Also highly similar to AAK46687|MT2395 drug transporter from Mycobacterium tuberculosis strain CDC1551 (537 aa), FASTA scores: opt: 1396, E(): 5.6e-74, (44.45% identity in 504 aa overlap); and P71879|Rv2333c|MTCY3G12.01 probable conserved integral membrane transport protein from Mycobacterium tuberculosis strain H37Rv (537 aa), FASTA scores: opt: 1385, E(): 2.5e-73, (44.25% identity in 504 aa overlap). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2760854 | 2762380 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2459|Rv2459
MTPRQRLTVLATGLGIFMVFVDVNIVNVALPSIQKVFHTGEQGLQWAVAGYSLGMAAVLMSCALLGDRYGRRRSFVFGVTLFVVSSIVCVLPVSLAVFTVARVIQGLGAAFISVLSLALLSHSFPNPRMKARAISNWMAIGMVGAASAPALGGLMVDGLGWRSVFLVNVPLGAIVWLLTLVGVDESQDPEPTQLDWVGQLTLIPAVALIAYTIIEAPRFDRQSAGFVAALLLAAGVLLWLFVRHEHRAAFPLVDLKLFAEPLYRSVLIVYFVVMSCFFGTLMVITQHFQNVRDLSPLHAGLMMLPVPAGFGVASLLAGRAVNKWGPQLPVLTCLAAMFIGLAIFAISMDHAHPVALVGLTIFGAGAGGCATPLLHLGMTKVDDGRAGMAAGMLNLQRSLGGIFGVAFLGTIVAAWLGAALPNTMADEIPDPIARAIVVDVIVDSANPHAHAAFIGPGHRITAAQEDEIVLAADAVFVSGIKLALGGAAVLLTGAFVLGWTRFPRTPAS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
