Gene Rv2505c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involved in lipid degradation. |
| Product | Probable fatty-acid-CoA ligase FadD35 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) |
| Comments | Rv2505c, (MTCY07A7.11c), len: 547 aa. Probable fadD35, fatty-acid-CoA synthetase, highly similar to many e.g. Q9Z5A6|SC2G5.17 from Streptomyces coelicolor (541 aa), FASTA scores: opt: 2202, E(): 8e-131, (61.55% identity in 528 aa overlap); Q9F9U4|FADD from Pseudomonas stutzeri (Pseudomonas perfectomarina), FASTA scores: opt: 1551, E(): 7.3e-90, (55.55% identity in 551 aa overlap); Q987S7|MLR6932 from Rhizobium loti (Mesorhizobium loti) (590 aa), FASTA scores: opt: 1453, E(): 1.1e-83, (50.7% identity in 564 aa overlap); etc. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2819953 | 2821596 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2505c|fadD35
VAAAEVVDPNRLSYDRGPSAPSLLESTIGANLAATAARYGHREALVDMVARRRFNYSELLTDVHRLATGLVRAGIGPGDRVGIWAPNRWEWVLVQYATAEIGAILVTINPAYRVREVEYALRQSGVAMVIAVASFKDADYAAMLAEVGPRCPDLADVILLESDRWDALAGAEPDLPALQQTAARLDGSDPVNIQYTSGTTAYPKGVTLSHRNILNNGYLVGELLGYTAQDRICIPVPFYHCFGMVMGNLAATSHGAAMVIPAPGFDPAATLRAVQDERCTSLYGVPTMFIAELGLPDFTDYELGSLRTGIMAGAACPVEVMRKVISRMHMPGVSICYGMTETSPVSTQTRADDSVDRRVGTVGRVGPHLEIKVVDPATGETVPRGVVGEFCTRGYSVMAGYWNDPQKTAEVIDADGWMHTGDLAEMDPSGYVRIAGRIKDLVVRGGENISPREIEELLHTHPDIVDGHVIGVPDAKYGEELMAVVKLRNDAPELTIERLREYCMGRIARFKIPRYLWIVDEFPMTVTGKVRKVEMRQQALEYLRGQQ
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
