Gene Rv2522c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv2522c, (MTV009.07c), len: 470 aa. Conserved hypothetical protein, equivalent, but longer 20 aa, to Q9X7E4|ML1193|MLCB458.08 from hypothetical 46.6 KDA protein Mycobacterium leprae (442 aa), FASTA scores: opt: 2521, E(): 4.1e-142, (86.35% identity in 440 aa overlap). Also similar to various proteins e.g. Q9K425|SCG22.20 putative peptidase from Streptomyces coelicolor (451 aa), FASTA scores: opt: 1097, E(): 1.1e-57, (42.5% identity in 451 aa overlap); Q9FCK3|2SC3B6.09 putative peptidase from Streptomyces coelicolor (470 aa), FASTA scores: opt: 669, E(): 2.8e-32, (34.2% identity in 462 aa overlap); Q98AF9|MLL6018 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (486 aa), FASTA scores: opt: 622, E(): 1.7e-29, (33.95% identity in 442 aa overlap); Q9RSU7|DR2025 ARGE/DAPE/ACY1 family protein from Deinococcus radiodurans (459 aa), FASTA scores: opt: 616, E(): 3.7e-29, (34.15% identity in 442 aa overlap); etc (include some similarity to hypothetical proteins from C. elegans and yeast). Alternative start possible at 6687 but then no RBS obvious. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2838129 | 2839541 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2522c|Rv2522c
VSASRRRIASKSGFSCDSASARELVERVREVLPSVRCDLEELVRIESVWADPDRRDEVHRSARAVADLLSQAGFDDVRIVSERGAPAVIARYPAPPGAPTVLLYAHHDVQPEGDRGQWVSPPFEPTERGGRLYGRGTADDKAGIATHVAAFWAHGGRPPVGVTVFVEGEEESGSPSLGRLLAAHRDALAADVIVIADSDNWSTDIPALTVSLRGMADCVVEVATLDHGLHSGLWGGVVPDALTVLVRLLASLHDDDGNVAVAGMHESTAARVDYPAGRVRAESGLLDGVSEIGTGSVPQRLWAKPAITVIGIDTTSVAAASNTLIPRARAKISIRVAPGGDATAHLDAVEAHLRRHAPWGAQVTVTRGEVGQPYAIEASGPVYDAARSAFRQAWGADPIDMGMGGSIPFIAEFAAAFPQATILVTGVEDPGTQAHSVNESLHLGVLERAATAEALLLAKLAAIPTGRAEA
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
