Gene Rv2537c (aroQ)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved at the third step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway). Catalyze a trans-dehydration via an enolate intermediate [catalytic activity: 3-dehydroquinate = 3-dehydroshikimate + H(2)O]. |
| Product | 3-dehydroquinate dehydratase AroD (AROQ) (3-dehydroquinase) (type II dhqase) |
| Comments | Rv2537c, (MTCY159.19), len: 147 aa. AroD (alternate gene name: aroQ), 3-dehydroquinate dehydratase (see citation below), equivalent to Q9CCS3|AROD|ML0519 3-dehydroquinate dehydratase from Mycobacterium leprae (145 aa), FASTA scores: opt: 803, E(): 3.4e-46, (85.9% identity in 142 aa overlap). Also highly similar to many e.g. P96750|AROQ_CORPS from Corynebacterium pseudotuberculosis (146 aa), FASTA scores: opt: 559, E(): 4.1e-30, (61.05% identity in 136 aa overlap); Q9K949|BH2801 from Bacillus halodurans (145 aa), FASTA scores: opt: 453, E(): 4e-23, (52.15% identity in 138 aa overlap); P54517|AROQ_BACSU|YQHS from Bacillus subtilis (148 aa), FASTA scores: opt: 419, E(): 7.1e-21, (45.3% identity in 139 aa overlap); etc. Contains PS01029 Dehydroquinase class II signature. Belongs to the type-II 3-dehydroquinase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2861148 | 2861591 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2537c|aroD
MSELIVNVINGPNLGRLGRREPAVYGGTTHDELVALIEREAAELGLKAVVRQSDSEAQLLDWIHQAADAAEPVILNAGGLTHTSVALRDACAELSAPLIEVHISNVHAREEFRRHSYLSPIATGVIVGLGIQGYLLALRYLAEHVGT
Bibliography
- Garbe T et al. [1991]. The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases. Sequence
- Moore JD et al. [1992]. Inducible overproduction of the Aspergillus nidulans pentafunctional AROM protein and the type-I and -II 3-dehydroquinases from Salmonella typhi and Mycobacterium tuberculosis. Product Biochemistry
- Gourley DG et al. [1994]. Crystallization of a type II dehydroquinase from Mycobacterium tuberculosis. Product Structure
- Gourley DG et al. [1999]. The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction. Product Structure
- Evans LD et al. [2002]. Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions. Structure
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Proteogenomic analysis of polymorphisms and gene annotation divergences in prokaryotes using a clustered mass spectrometry-friendly database. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
