Go to browser
virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved at the third step in the biosynthesis of chorismate within the biosynthesis of aromatic amino acids (the shikimate pathway). Catalyze a trans-dehydration via an enolate intermediate [catalytic activity: 3-dehydroquinate = 3-dehydroshikimate + H(2)O].
Product3-dehydroquinate dehydratase AroD (AROQ) (3-dehydroquinase) (type II dhqase)
CommentsRv2537c, (MTCY159.19), len: 147 aa. AroD (alternate gene name: aroQ), 3-dehydroquinate dehydratase (see citation below), equivalent to Q9CCS3|AROD|ML0519 3-dehydroquinate dehydratase from Mycobacterium leprae (145 aa), FASTA scores: opt: 803, E(): 3.4e-46, (85.9% identity in 142 aa overlap). Also highly similar to many e.g. P96750|AROQ_CORPS from Corynebacterium pseudotuberculosis (146 aa), FASTA scores: opt: 559, E(): 4.1e-30, (61.05% identity in 136 aa overlap); Q9K949|BH2801 from Bacillus halodurans (145 aa), FASTA scores: opt: 453, E(): 4e-23, (52.15% identity in 138 aa overlap); P54517|AROQ_BACSU|YQHS from Bacillus subtilis (148 aa), FASTA scores: opt: 419, E(): 7.1e-21, (45.3% identity in 139 aa overlap); etc. Contains PS01029 Dehydroquinase class II signature. Belongs to the type-II 3-dehydroquinase family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS28611482861591-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2537c|aroD
MSELIVNVINGPNLGRLGRREPAVYGGTTHDELVALIEREAAELGLKAVVRQSDSEAQLLDWIHQAADAAEPVILNAGGLTHTSVALRDACAELSAPLIEVHISNVHAREEFRRHSYLSPIATGVIVGLGIQGYLLALRYLAEHVGT
      
Bibliography