Gene Rv2542
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv2542, (MTCY159.14c), len: 403 aa. Conserved hypothetical protein, highly similar to AAK46927|MT2616 hypothetical 28.0 KDA protein from Mycobacterium tuberculosis strain CDC1551 (265 aa), FASTA scores: opt: 1776, E(): 2.3e-94, (99.25% identity in 265 aa overlap). And similar to several hypothetical proteins from Mycobacterium tuberculosis (strain H37Rv and CDC1551) e.g. P71654|Rv2797c|MTCY16B7.46 (562 aa), FASTA scores: opt: 537, E(): 2.6e-23, (40.75% identity in 292 aa overlap); P71547|Y963_MYCTU|Rv0963c|MT0992|MTCY10D7.11 (266 aa), FASTA scores: opt: 357, E(): 2.6e-13, (34.6% identity in 234 aa overlap); Q10685|YK77_MYCTU|Rv2077c|MT2137|MTCY49.16c (323 aa), FASTA scores: opt: 261, E(): 9.5e-08, (32.7% identity in 211 aa overlap); etc. Also similar to Q9RDQ9|SC4A7.03 putative secreted protein from Streptomyces coelicolor (406 aa), FASTA scores: opt: 247, E(): 7.3e-07, (30.35% identity in 303 aa overlap). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2865130 | 2866341 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2542|Rv2542
VLDAVSDARRDGFAVGEDYTVTDRSTGGSRQQRAARLGQAQGHADFIRHRVGALLATDRDIATRVSAATQGLDELAFEDVPGVDTPAEDGVQAVDFRQAPPPGAPGGMSSGDIDAIDAANRALLQDMLAEYSRLPDGQVKTDRLADIAAIQEALRVPDSHLIYVARPDDPADMIPAVTAVGDPFTADHVSVTVPGVSGTTRQTIATMTQETRGLREEARVIAHSVGESENVATIAWVGYQPPPVLASWNTVDDDLAQAGAPKLEAFLRDLQAGSHNPGHTTALFGHSYGSLLSGIALKDGASSLVDNAVLYGSPGFDATSPAKLGMNDHNFFVMTTPDDPIRYPARLAPLHGWGSDGADTIGTVGRQGTPARVGIRPQRDHRRIPGPLPLHPSADRRGIHSAG
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
