Gene Rv2566
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Long conserved protein |
| Comments | Rv2566, (MTCY9C4.02c), len: 1140 aa. Long conserved protein, equivalent to O53120|ML2678 or MLCB1913.12 hypothetical protein from Mycobacterium leprae (1000 aa), FASTA scores: opt: 760, E(): 7.1e-38, (50.2% identity in 1128 aa overlap); and middle part equivalent to Q9ZB40 72.2 KDA protein (fragment) from Mycobacterium leprae (644 aa), FASTA scores: opt: 1017, E(): 1.5e-65, (45.65% identity in 655 aa overlap). Also highly similar to Q98HG6|MLL2877 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (1119 aa), FASTA scores: opt: 1413, E(): 3.7e-77, (52.4% identity in 1148 aa overlap); and N-terminus shows similarity with other proteins e.g. Q9HUN8|PA4926 hypothetical protein from Pseudomonas aeruginosa (311 aa), FASTA scores: opt: 278, E(): 3e-09, (29.95% identity in 284 aa overlap); and upstream ORF Q50652|YP69_MYCTU|Rv2569c|MT2645|MTCY227.32 conserved hypothetical protein from Mycobacterium tuberculosis (314 aa), FASTA scores: opt: 252, E(): 1.1e-07, (28.9% identity in 315 aa overlap). Equivalent to AAK46955 from Mycobacterium tuberculosis strain CDC1551 (1156 aa) but shorter 16 aa. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 and 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2886373 | 2889795 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2566|Rv2566
VPLRPTQVSGTGRTRCAGRSGVISSAAMSIKVALEHRTSYTFDRLVRVYPHIVRLRPAPHSRTSIEAYSLRIEPADHFINWQQDALGNFLARLVFPNPMRQLRITVGLIADLKVINPFDFFIEDWAEIWPCAGMAYPKALADDLRPYLRPVDEDGDGSGPGELTQAWVRNFTVPDGTRTIDFLVALNRAINADVGYCVRMEPGVQTPDFTLRTGVGSCRDSAWLLVSILRQFGLAARFVSGYLVQLASDIEALDGPSGPAADFTDLHAWAEAYIPGAGWIGLDPTSGLLAGEGHIPLAATPHPASAAPISGGTDVCDTVLEFSNTVTRVHEDPRVTLPYTDESWKTICEVGQRVDERLAAADVRLTVGGEPTFVSVDNQVAEEWRTAADGPHKRERASDLAARLKAVWAPQGLIHRGQGRWYPGEPLPRWQIALYWRTDGRPLWTNDALLADPWGAPPADPVDDDAAYRVLAGIADGLGLPISQVRPAYEDPLSRLAAAVRMPAGDPVESGDDLGCDTNPDTPTGRAALLARLDEAITSPAAYVLPLHRRDDGQGWASANWRLRRGRIVLLEGDSPAGLRLPLDSISWRPPRASFDADPVAVRSTLPAELHTDRAVVEDPETAPTTALVAEVRGGLVHIFLPPTDALEHFIDLVARVEAAATTANCPVVIEGYGPPPDPRLTSTTITPDPGVIEVNIAPTASFAEQRQQLETLYQQARLARLTTEAFDVDGTHGGTGGGNHITLGGVTPADSPLLRRPDLLVSLLTYWQRHPSLSYLFAGRFVGTTSQAPRVDEGRAEALYELEIAFAEILRLSPSSGGGRPQPWVTDRALRHLLTDITGNTHRAEFCIDKLYSPDSARGRLGLLELRGFEMPPHLHMAMVQSLLVRSLVAWFWDQPLRAPLIRHGANLHGRYLLPHFLIHDIADVAADLRAHGIAFETSWLDPFTEFRFPRIGTAVFDGIEIELRGAIEPWHTLGEEATAAGTARYVDSSVERIQVRIIGADRHRYVVTCNGYPMPLLATDNPDIHVGGVRFKAWQPPSALHPTITVDGPLRFELIDIATATSCGGCTYHVAHPGGRAYDEPPVNAVEAEARRARRFEATGFTPGKLDLSDIREKQARISTDIGAPGILDLRRVRTVQQ
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
