Gene Rv2569c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv2569c, (MTCY227.32), len: 314 aa. Conserved hypothetical protein, equivalent to Q9CCT2|ML0508 hypothetical protein from Mycobacterium leprae (313 aa), FASTA scores: opt: 1723, E(): 1.9e-95, (84.4% identity in 301 aa overlap); and some similarity with Q49757|YP69_MYCLE|ML0607|MLCL536.03c|B1937_F2_39 hypothetical 31.1 KDA protein from Mycobacterium leprae (279 aa), FASTA scores: opt: 305, E(): 4.5e-11, (33.0% identity in 300 aa overlap). Also similar to to other hypothetical proteins e.g. Q9HUN8|PA4926 from Pseudomonas aeruginosa (311 aa), FASTA scores: opt: 704, E(): 8.7e-35, (39.7% identity in 320 aa overlap); Q98HG8|MLL2875 from Rhizobium loti (Mesorhizobium loti) (294 aa), FASTA scores: opt: 521, E(): 6.5e-24, (35.05% identity in 294 aa overlap); Q9A7W9|CC1600 from Caulobacter crescentus (325 aa), FASTA scores: opt: 510, E(): 3.2e-23, (34.4% identity in 2588 aa overlap); etc. Also some similarity with proteins from Mycobacterium tuberculosis e.g. P71734|Rv2409c|MTCY253.11 conserved hypothetical protein (279 aa), FASTA scores: opt: 312, E(): 1.7e-11, (34.45% identity in 296 aa overlap); and Q50732|Rv2566|MTCY9C4.02 long conserved hypothetical protein (1140 aa), FASTA scores: opt: 252, E(): 2.2e-07, (28.9% identity in 315 aa overlap) |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2893464 | 2894408 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2569c|Rv2569c
LSADSSLSLPLSGTHRYRVTHRTEYRYSDVVTSSYGRGFLTPRNSLRQRCVAHRLTIDPAPADRSTSRDGYGNISSYFHVTEPHRTLTITSDSIVDVSPPPPGLYTSGPALQPWEAARPAGLPGSLATEFTLDLNPPEITDAVREYAAPSFLPKRPLVEVLRDLASRIYTDFTYRSGSTTISTGVNEVLLAREGVCQDFARLAIACLRANGLAACYVSGYLATDPPPGKDRMIGIDATHAWASVWTPQQPGRFEWLGLDPTNDQLVDQRYIVVGRGRDYADVPPLRGIIYTNSENSVIDVSVDVVPFEGDALHA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
