Gene Rv2604c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in the biosynthesis of pyridoxine/pyridoxal 5-phosphate biosynthesis |
| Product | Probable glutamine amidotransferase SnoP |
| Comments | Rv2604c, (MTCY01A10.29, MTCI270A.01), len: 198 aa. Probable snoP, glutamine amidotransferase, equivalent (but shorter 21 aa) to Q49637|HISH|B1177_C1_149 HISH protein (belongs to the YFL060C/YAAE/HI1648 family) (alias Q9CCT5|ML0474 hypothetical protein 223 aa) from Mycobacterium leprae (219 aa), FASTA scores: opt: 1069, E(): 1.7e-60, (83.35% identity in 198 aa overlap). Also highly similar to hypothetical proteins or amidotransferases e.g. Q9L287|SCL2.12c hypothetical 21.5 KDA protein from Streptomyces coelicolor (202 aa), FASTA scores: opt: 702, E(): 2.3e-37, (56.75% identity in 192 aa overlap); P37528|YAAE_BACSU hypothetical 21.4 KDA protein from Bacillus subtilis (196 aa), FASTA scores: opt: 608, E(): 1.9e-31, (48.7% identity in 189 aa overlap); Q9KGN5|BH0023 amidotransferase from Bacillus halodurans (196 aa), FASTA scores: opt: 583, E(): 7.4e-30, (48.7% identity in 195 aa overlap); etc. Also some similarity with several proteins from Mycobacterium tuberculosis e.g. O06589|HIS5_MYCTU|Rv1602|MT1638|MTCY336.02c amidotransferase (206 aa), FASTA scores: opt: 154, E(): 0.00036, (30.6% identity in 193 aa overlap). Contains a Pfam match to entry PF01174 SNO glutamine amidotransferase family. Note possibly co-regulated with snzP (Rv2606c). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2931693 | 2932289 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2604c|snoP
VSVPRVGVLALQGDTREHLAALRECGAEPMTVRRRDELDAVDALVIPGGESTTMSHLLLDLDLLGPLRARLADGLPAYGSCAGMILLASEILDAGAAGRQALPLRAMNMTVRRNAFGSQVDSFEGDIEFAGLDDPVRAVFIRAPWVERVGDGVQVLARAAGHIVAVRQGAVLATAFHPEMTGDRRIHQLFVDIVTSAA
Bibliography
- Mittenhuber G [2001]. Phylogenetic analyses and comparative genomics of vitamin B6 (pyridoxine) and pyridoxal phosphate biosynthesis pathways. Homolog Review
- Gao LY et al. [2003]. Transposon mutagenesis of Mycobacterium marinum identifies a locus linking pigmentation and intracellular survival. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
