Gene Rv2613c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; but could be involved in lipid metabolism. |
| Product | Conserved protein |
| Comments | Rv2613c, (MTCY01A10.20A), len: 195 aa. Conserved protein, equivalent to Q9CCU0|ML0455 hypothetical protein from Mycobacterium leprae (206 aa), FASTA scores: opt: 1074, E(): 7.4e-62, (84.7% identity in 196 aa overlap); and highly similar, but longer 18 aa, to O07150|MLCL581.17c hypothetical 20.7 KDA protein from Mycobacterium leprae (186 aa), FASTA scores: opt: 1038, E(): 1.4e-59, (89.7% identity in 175 aa overlap). Also highly similar to other hypothetical proteins (often Hit family member) e.g. Q9F7Z0 from Mycobacterium smegmatis (see citation below) (205 aa), FASTA scores: opt: 975, E(): 1.6e-55, (79.35% identity in 184 aa overlap); Q9L279|SCL2.20 from Streptomyces coelicolor (186 aa), FASTA scores: opt: 638, E(): 5.8e-34, (52.85% identity in 176 aa overlap); Q9YFX8|APE0122 from Aeropyrum pernix (184 aa), FASTA scores: opt: 515, E(): 4.4e-26, (45.9% identity in 159 aa overlap); etc. It seems the Rv2613c and downstream ORF Rv2612c|psgA1 are expressed from the same promoter (see citation below) and that Rv2613c should be involved in lipid metabolism. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al.,2003). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2940609 | 2941196 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2613c|Rv2613c
VSDEDRTDRATEDHTIFDRGVGQRDQLQRLWTPYRMNYLAEAPVKRDPNSSASPAQPFTEIPQLSDEEGLVVARGKLVYAVLNLYPYNPGHLMVVPYRRVSELEDLTDLESAELMAFTQKAIRVIKNVSRPHGFNVGLNLGTSAGGSLAEHLHVHVVPRWGGDANFITIIGGSKVIPQLLRDTRRLLATEWARQP
Bibliography
- Jackson M et al. [2000]. Phosphatidylinositol is an essential phospholipid of mycobacteria. Homolog Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
