Gene Rv2673
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the biosynthesis of the mycobacterial cell wall arabinan |
| Product | Possible arabinofuranosyltransferase AftC |
| Comments | Rv2673, (MTCY441.42), len: 433 aa. Possible aftC, arabinofuranosyltransferase (See Birch et al., 2008). Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). Possible conserved integral membrane protein, equivalent to MLC1351.25|ML1338 possible conserved integral membrane protein from Mycobacterium leprae (440 aa), FASTA scores: opt: 2410, E(): 5.3e-143, (82.05% identity in 434 aa overlap); and showing some similarity with Q9CBX0|ML1504 probable conserved membrane protein from Mycobacterium leprae (430 aa), FASTA scores: opt: 159, E(): 0.014, (24.4% identity in 340 aa overlap). Also similar to Q53873|SC6G4.11 putative integral membrane protein from Streptomyces coelicolor (411 aa), FASTA scores: opt: 383, E(): 1.4e-16, (29.6% identity in 422 aa overlap); and with weak similarity with P71061|YVFB hypothetical protein from Bacillus subtilis (396 aa), FASTA scores: opt: 136, E(): 0.36, (24.35% identity in 279 aa overlap); and BAB60134|TVG1014811 hypothetical protein from Thermoplasma volcanium (695 aa), FASTA scores: opt: 133, E(): 0.85, (26.45% identity in 280 aa overlap). Shows also some similarity with O06557|Rv1159|MTCI65.26 hypothetical 47.1 KDA protein from Mycobacterium tuberculosis (431 aa), FASTA scores: opt: 149, E(): 0.059, (22.45% identity in 410 aa overlap); and O53515|Rv2181|MTV021.14 putative membrane protein from Mycobacterium tuberculosis (427 aa), FASTA scores: opt: 129, E(): 1, (24.8% identity in 367 aa overlap). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al.,2003). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2989291 | 2990592 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2673|aftC
VYGALVTAADSIRTGLGASLLAGFRPRTGAPSTATILRSALWPAAVLSVLHRSIVLTTNGNITDDFKPVYRAVLNFRRGWDIYNEHFDYVDPHYLYPPGGTLLMAPFGYLPFAPSRYLFISINTAAILVAAYLLLRMFNFTLTSVAAPALILAMFATETVTNTLVFTNINGCILLLEVLFLRWLLDGRASRQWCGGLAIGLTLVLKPLLGPLLLLPLLNRQWRALVAAVVVPVVVNVAALPLVSDPMSFFTRTLPYILGTRDYFNSSILGNGVYFGLPTWLILFLRILFTAITFGALWLLYRYYRTGDPLFWFTTSSGVLLLWSWLVMSLAQGYYSMMLFPFLMTVVLPNSVIRNWPAWLGVYGFMTLDRWLLFNWMRWGRALEYLKITYGWSLLLIVTFTVLYFRYLDAKADNRLDGGIDPAWLTPEREGQR
Bibliography
- Liu J et al. [2003]. Three monophyletic superfamilies account for the majority of the known glycosyltransferases. Homology
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Birch HL et al. [2008]. Biosynthesis of mycobacterial arabinogalactan: identification of a novel alpha(1-->3) arabinofuranosyltransferase. Function Product
- Birch HL et al. [2010]. A truncated lipoglycan from mycobacteria with altered immunological properties. Function Product
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
