Gene Rv2679
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Could possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O]. |
| Product | Probable enoyl-CoA hydratase EchA15 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase) |
| Comments | Rv2679, (MTV010.03), len: 276 aa. Probable echA15, enoyl-CoA hydratase, similar to P53526|ECHC_MYCLE|ECHA12|ML1241|MLCB1610.01|B1170_C2_224 probable enoyl-CoA hydratase from Mycobacterium leprae (294 aa), FASTA scores: opt: 368, E(): 2.5e-16, (32.15% identity in 277 aa overlap). Also highly similar to Q9RXX1|DR0184 from Deinococcus radiodurans (273 aa), FASTA scores: opt: 993, E(): 2.2e-56, (58.15% identity in 263 aa overlap); and similar to many e.g. Q9ETY7|PACA|PAAG from Azoarcus evansii (273 aa), FASTA scores: opt: 396, E(): 3.8e-18, (34.9% identity in 258 aa overlap); O29299|AF0963|FAD-3 from Archaeoglobus fulgidus (259 aa), FASTA scores: opt: 363, E(): 4.7e-16, (30.4% identity in 250 aa overlap); P77467|PAAG_ECOLI|B1394 from Escherichia coli strain W (262 aa), FASTA scores: opt: 357, E(): 1.1e-15, (31.75% identity in 252 aa overlap); etc. Also similar to O53163|ECHC_MYCTU|ECHA12|FADB2|Rv1472|MT1518|MTV007.19 enoyl-CoA hydratase from Mycobacterium tuberculosis (285 aa), FASTA scores: opt: 355, E(): 1.6e-15, (31.3% identity in 265 aa overlap); and O06542|ECHA10|Rv1142c|MTCI65.09c|Z95584 enoyl-CoA hydratase from Mycobacterium tuberculosis (268 aa). Contains PS00166 Enoyl-CoA hydratase/isomerase signature. Belongs to the enoyl-CoA hydratase/isomerase family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in the culture filtrate and whole cell lysates of M. tuberculosis H37Rv but not the membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2995115 | 2995945 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2679|echA15
MPVTYDDFPSLRCEIHDQPGHEGVLELVLDSPGLNSVGPHMHRDLADIWPVIDRDPAVRVVLVRGEGKAFSSGGSFDLIAETIGDYQGRLRIMREARDLVLNLVNFDKPVVSAIRGPAVGAGLVVALLADISVAGRAAKIIDGHTKLGVAAGDHAAICWPLLVGMAKAKYYLLTCEPLSGEEAERIGLVSICVDDDDVLPTATRLAERLAAGAQNAIRWTKRSLNHWYRMFGPAFETSLGLEFIGFGGPDVREGLAAHREKRPARFGADPDPGAGS
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Proteogenomic analysis of polymorphisms and gene annotation divergences in prokaryotes using a clustered mass spectrometry-friendly database. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
