Gene Rv2685 (arsB)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in transport of arsenical compounds across the membrane (export): arsenic resistance by an export mechanism. Responsible for the translocation of the substrate across the membrane. |
| Product | Probable arsenic-transport integral membrane protein ArsB1 |
| Comments | Rv2685, (MTCY05A6.06), len: 428 aa. Probable arsB1, arsenic-transport integral membrane protein, equivalent to P46838|AG45_MYCLE|ML1036 46 KDA probable integral membrane protein (antigen 45, a transmembrane protein related to arsenical pumps) from Mycobacterium leprae (429 aa), FASTA scores: opt: 2048, E(): 7.3e-120, (74.25% identity in 427 aa overlap); and downstream ORF O07186|YQ84_MYCTU|ARSA|Rv2684|MT2758|MTCY05A6.05 probable integral membrane protein ARSA from Mycobacterium tuberculosis (429 aa), FASTA scores: opt: 2154, E(): 1.9e-126, (76.8% identity in 427 aa overlap). Also highly similar to other proteins e.g. O59575|PH1912 hypothetical 46.0 KDA protein from Pyrococcus horikoshii (424 aa), FASTA scores: opt: 1075, E(): 1.9e-59, (43.55% identity in 427 aa overlap); Q9UY19|PAB1107 transport protein from Pyrococcus abyssi (425 aa), FASTA scores: opt: 1062, E(): 1.3e-58, (41.8% identity in 428 aa overlap); Q9KDI2|BH1231 hypothetical 46.0 KDA protein from Bacillus halodurans (428 aa), FASTA scores: opt: 993, E(): 2.4e-54, (39.55% identity in 430 aa overlap); etc. Belongs to the NADC/P/PHO87 family of transporters, P subfamily. Note that previously known as arsB. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3001983 | 3003269 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2685|arsB1
MSIIAITVFVAGYALIASDRVSKTRVALTCAAIMVGAGIVGSDDVFYSHEAGIDWDVIFLLLGMMIIVSVLRHTGVFEYVAIWAVKRANAAPLRIMILLVLVTALGSALLDNVTTVLLIAPVTLLVCDRLGVNSTPFLVAEVFASNVGGAATLVGDPPNIIIASRAGLTFNDFLIHMAPAVLVVMIALIGLLPWLLGSVTAEPDRVADVLSLNEREAIHDRGLLIKCGVVLVLVFAAFIAHPVLHIQPSLVALLGAGVLVRFSGLERSDYLSSVEWDTLLFFAGLFVMVGALVKTGVVEQLARAATELTGGNELLTVGLILGISAPVSGIIDNIPYVATMTPIVTELVAAMPGHVHPDTFWWALALSADFGGNLTAVAASANVVMLGIARRSGTPISFWKFTRKGAVVTAVSLVLSAVYLWLRYFVFG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
